CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
转晚期胚胎发生丰富蛋白(LEA)基因棉花及抗旱性分析/Transgenic Cotton with Late Embryogenesis Abundant Protein (LEA) Gene and its Drought Tolerance[J]
Authors
Cai Ming
Li Chunping
+14 more
Li Xiaoshuang
Pei Jingling
Yang Honglan
Zhang Daoyuan
Zhang Dawei
中国科学院新疆生态与地理研究所,乌鲁木齐,830011
张大伟
张道远
新疆农科院经济作物研究所,乌鲁木齐,830000
李小双
李春平
杨红兰
蔡明
裴金玲
Publication date
1 January 2012
Publisher
Abstract
本研究将紫杆柽柳(Tamarix and rossowii Litv)晚期胚胎发生丰富蛋白(LEA DQ663481)基因导入新疆早熟棉新陆早18号,通过冬季海南加代、夏季新疆继代的方法获得T3代转基因棉花株系.从40株大田卡那霉素检测阳性植株中,经过PCR筛选证明3株为阳性的转化株.在室内条件下,对转化株幼苗进行水培实验,并用12% (w/v) PEG-6000处理12h模拟干旱胁迫.结果显示,干旱胁迫之后,转LEA基因棉花基因表达量显著增加;丙二醛生成量显著降低;游离脯氨酸、可溶性糖、可溶性蛋白的生成量显著增加;棉花表型分析也证明了转LEA基因棉花抗旱性有提高.培养45d后,转LEA基因棉花的生物量高于非转基因棉花.本研究结果表明,在干旱胁迫条件下,转LEA基因棉花表现出了优良的生长和生理优势,显示出转LEA基因棉花能提高棉花的抗旱能力
Similar works
Full text
Available Versions
Institutional Repository of Xinjiang Institute of Ecology and Geography, CAS
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.xjlas.org:365004/13388
Last time updated on 29/11/2016