Hovatta, Outi

Inzunza, Jose

Katayama, Shintaro

Kere, Juha

Madissoon, Elo

Tohonen, Virpi

Unneberg, Per

Vesterlund, Liselotte

PLoS ONE

PubMed

Infertility is a worldwide concern that can be treated with in vitro fertilization (IVF). Improvements in IVF and infertility treatment depend largely on better understanding of the molecular mechanisms for human preimplantation development. Several large-scale studies have been conducted to identify gene expression patterns for the first five days of human development, and many functional studies utilize mouse as a model system. We have identified genes of possible importance for this time period by analyzing human microarray data and available data from online databases. We selected 70 candidate genes for human preimplantation development and investigated their expression in the early mouse development from oocyte to the 8-cell stage. Maternally loaded genes expectedly decreased in expression during development both in human and mouse. We discovered that 25 significantly upregulated genes after fertilization in human included 13 genes whose orthologs in mouse behaved differently and mimicked the expression profile of maternally expressed genes. Our findings highlight many significant differences in gene expression patterns during mouse and huma

Elo Madissoon

Liselotte Vesterlund

Shintaro Katayama

Per Unneberg

Jose Inzunza

Outi Hovatta

Juha Kere

CiteSeerX

Differences in Gene Expression between Mouse and Human for Dynamically Regulated Genes in Early Embryo

Helsingin yliopiston digitaalinen arkisto

Infertility is a worldwide concern that can be treated with in vitro fertilization (IVF). Improvements in IVF and infertility treatment depend largely on better understanding of the molecular mechanisms for human preimplantation development. Several large-scale studies have been conducted to identify gene expression patterns for the first five days of human development, and many functional studies utilize mouse as a model system. We have identified genes of possible importance for this time period by analyzing human microarray data and available data from online databases. We selected 70 candidate genes for human preimplantation development and investigated their expression in the early mouse development from oocyte to the 8-cell stage. Maternally loaded genes expectedly decreased in expression during development both in human and mouse. We discovered that 25 significantly upregulated genes after fertilization in human included 13 genes whose orthologs in mouse behaved differently and mimicked the expression profile of maternally expressed genes. Our findings highlight many significant differences in gene expression patterns during mouse and human preimplantation development. We also describe four cancer-testis antigen families that are also highly expressed in human embryos: PRAME, SSX, GAGE and MAGEA

Virpi Töhönen

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Differences in gene expression between mouse and human for dynamically regulated genes in early embryo.

<div><p>Infertility is a worldwide concern that can be treated with <i>in vitro</i> fertilization (IVF). Improvements in IVF and infertility treatment depend largely on better understanding of the molecular mechanisms for human preimplantation development. Several large-scale studies have been conducted to identify gene expression patterns for the first five days of human development, and many functional studies utilize mouse as a model system. We have identified genes of possible importance for this time period by analyzing human microarray data and available data from online databases. We selected 70 candidate genes for human preimplantation development and investigated their expression in the early mouse development from oocyte to the 8-cell stage. Maternally loaded genes expectedly decreased in expression during development both in human and mouse. We discovered that 25 significantly upregulated genes after fertilization in human included 13 genes whose orthologs in mouse behaved differently and mimicked the expression profile of maternally expressed genes. Our findings highlight many significant differences in gene expression patterns during mouse and human preimplantation development. We also describe four cancer-testis antigen families that are also highly expressed in human embryos: PRAME, SSX, GAGE and MAGEA.</p></div

Elo Madissoon (608322)

Virpi Töhönen (249771)

Liselotte Vesterlund (409106)

Shintaro Katayama (13863)

Per Unneberg (3090)

Jose Inzunza (250616)

Outi Hovatta (51032)

Juha Kere (11900)

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https://figshare.com/articles/_Differences_in_Gene_Expression_between_Mouse_and_Human_for_Dynamically_Regulated_Genes_in_Early_Embryo_/1126546

Differences in Gene Expression between Mouse and Human for Dynamically Regulated Genes in Early Embryo

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Helsingin yliopiston digitaalinen arkisto

Directory of Open Access Journals

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