Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities

Abstract

为获得能明显抑制水华蓝藻的化感物质,本研究从28株大型海藻内共生真菌中筛选出了4株菌株,其粗提物能显著抑制水华鱼腥藻(Anabaena flos-aquae)和铜绿微囊藻(Microcystis aeruginosa)的生长。对这4株真菌扩大培养后,检测了从其次生代谢产物中分离出的32种单体化合物的抑藻活性。其中,化合物sterigmatocystin、(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene、 6,8-di-O-methylnidurufin和aversin对水华鱼腥藻的抑制率显著大于50%(P < 0.05),24 h半数有效浓度(EC_(50,24h))分别为6.865、4.302、1.824、2.319 μg/mL;化合物sterigmatocystin、indol-3-carboxaldehyde和(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene对铜绿微囊藻的抑制率显著大于50%(P < 0.05),EC_(50,24h)分别为4.167、5.374、8.386 μg/mL

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