Polo-like kinase 1 promotes assembly of the contractile ring that divides a cell in two by creating a docking site for the RhoA activator Ect2 on the Cyk-4-containing centralspindlin complex at the midzone of the mitotic spindle

A Piekny

A Santamaria

AE Elia

AJ Piekny

Benjamin A. Wolfe

C Mollinari

C Zhu

CK Hu

David Pellman

DM Lowery

F Niiya

IM Brennan

JB Keeney

JC Canman

JE Kim

JN Glover

K Kamijo

K Murthy

M Asiedu

M Glotzer

M Mishima

M Nousiainen

M Petronczki

M Steegmaier

M Werner

Mark Petronczki

ME Burkard

Michael Glotzer

MS Lee

O Yuce

PP D'Avino

R Ban

R Neef

TJ Lansing

Tohru Takaki

U Gruneberg

V Pavicic-Kaltenbrunner

W Jiang

WM Zhao

X Liu

Y Kurasawa

Y Nishimura

English

PubMed

To complete cell division with high fidelity, cytokinesis must be coordinated with chromosome segregation. Mammalian Polo-like kinase 1, Plk1, may function as a critical link because it is required for chromosome segregation and establishment of the cleavage plane following anaphase onset. A central spindle-localized pool of the RhoGEF Ect2 promotes activation of the small GTPase RhoA, which drives contractile ring assembly at the equatorial cortex. Here, we have investigated how Plk1 promotes the central spindle recruitment of Ect2. Plk1 phosphorylates the noncatalytic N terminus of the RhoGAP HsCyk-4 at the central spindle, creating a phospho-epitope recognized by the BRCA1 C-terminal (BRCT) repeats of Ect2. Failure to phosphorylate HsCyk-4 blocks Ect2 recruitment to the central spindle and the subsequent induction of furrowing. Microtubules, as well as the microtubule-associated protein (MAP) Prc1, facilitate Plk1 phosphorylation of HsCyk-4. Characterization of a phosphomimetic version of HsCyk-4 indicates that Plk1 promotes Ect2 recruitment through multiple targets. Collectively, our data reveal that formation of the HsCyk-4-Ect2 complex is subject to multiple layers of regulation to ensure that RhoA activation occurs between the segregated sister chromatids during anaphase

Benjamin A Wolfe

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PLoS Biology

Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation.

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Polo-Like Kinase 1 Directs Assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF Complex to Initiate Cleavage Furrow Formation

Wolfe Benjamin A.

Takaki Tohru

Petronczki Mark

Glotzer Michael

Public Library of Science (PLOS)

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https://figshare.com/articles/Polo_Like_Kinase_1_Directs_Assembly_of_the_HsCyk_4_RhoGAP_Ect2_RhoGEF_Complex_to_Initiate_Cleavage_Furrow_Formation/147627

Polo-Like Kinase 1 Directs Assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF Complex to Initiate Cleavage Furrow Formation

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