research
Prevenção de translocação bacteriana com β-(1-3)-D-glucana em isquemia e reperfusão intestinal em ratos
- Publication date
- Publisher
Abstract
To investigate the role of β-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines
secretion in rats submitted to small bowel ischemia/reperfusion injury. Methods: Five groups (n=10 each) of Wistar rats
were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and
group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2mg/Kg intramuscular.
Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using
radioactivity/count and colony forming units/g(CFU/g). Serum TNFα, IL-1β, IL-6, IL-10 were measured by ELISA. Results:
CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and
radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of
serum TNFα, IL-1β and, IL-6, compared to I/R untreated animals. Conclusion: The β-(1-3)-D-glucan modulated the
production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated
translocation of labelled bacteriaCNP