Bordalo, Adriano A.

Cerdà, Victor

Mesquita, Raquel B. R.

Rangel, António O. S. S.

Rangel, M.

Suárez, Ruth

English

Repositório Institucional da Universidade Católica Portuguesa

Green analytical automatic method for iron speciation in bathing water samples  Ruth Suárez1, Raquel B. R. Mesquita2,3, Maria Rangel4, Adriano A. Bordalo3, Víctor Cerdà1,*, António O. S. S. Rangel2  1 Laboratory of Environmental Analytical Chemistry – LQA2, University of the Balearic Islands, Palma de Mallorca, Spain 2 CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Porto, Portugal 3 Laboratory of Hydrobiology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Institute of Marine Research (CIIMAR), Universidade do Porto, Portugal 4 REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Portugal *victor.cerda@uib.es  The speciation of iron(II) and iron(III) in dynamic water systems usually implies the determination with highly toxic reagents. For this fact, a green analytical method based on sequential injection lab-on-valve (SI-LOV) solid-phase spectrophotometry for iron speciation in bathing water samples was developed. The spectrophotometric detection was based on the colorimetric reaction between iron(III) and the non-toxic reagent 3-hydroxy-4-pyridinone (3,4-HPO) chelator, which are well known, mainly reason of their biomedical applications; they are particularly attractive for pharmaceutical purposes since their structure allows tailoring of their hydrophilic/lipophilic balance without significantly its chelating properties. To achieve a suitable limit of detection, a pre-concentration step was included using the Nitrilotriacetic Acid Superflow (NTA) resin. The NTA resin was placed in the flow cell for assembling the preconcentration column where iron was retained prior to the reaction with 3,4-HPO. Thus, the change of absorbance was monitored at 460 nm, corresponding to the complex formation. With this approach, both the low levels of iron in natural waters, and minimization of the high salinity interference in coastal water samples were attained. The developed method enabled a limit of detection of 3 µg Fe L−1 with a linear range between 20 - 100 µg Fe L−1, with low sample consumption and effluent production, 400 μL and 2.15 mL respectively. The proposed method was effectively applied to the speciation of iron in different bathing waters, coastal and inland.  Keywords: Iron speciation, SI-LOV, 3-hydroxy-4-pyridinone, green method, bathing water samples  Acknowledgements R. Suárez thank to Conselleria d'Educació, Cultura i Universitats from the Government of the Balearic Islands for allocation of a PhD stipend cofinanced by Fondo Social Europeo and to Ministerio de Educación Cultura e Deporte for the grant MHE2011-00034. R. B. R. Mesquita thank to Fundação para a Ciência e a Tecnologia (FCT) e Fundo Social Europeu through the program POPH – QREN for the grant SFRH/BPD/41859/2007, respectively. R. Suárez and V. Cerdà acknowledge financial support from Ministerio de Ciencia e Innovación throught Project CTQ2010-15541 and from Conselleria d’Economia, Hisenda, i Innovació of the Government of the Balearic Islands through the allowance to competitive groups (43/2011). This work was supported by National Funds through FCT the projects PEst-C/EQB/LA0016/2011 and PEst-C/EQB/LA0006/2011 and European Union FEDER funds through COMPETE and by projects PTDC/AAC-AMB/104882/2008 and PTDC/AAG-MAA/3978/2012. 

Green analytical automatic method for iron speciation in bathing water samples

https://repositorio.ucp.pt/bitstream/10400.14/18975/1/2013_IQIV_Ruth.pdf

Green analytical automatic method for iron speciation in bathing water samples

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Repositório Institucional da Universidade Católica Portuguesa