Agency of Innovation (Agência  de  Inovação,  ADI, Portugal) and Quadro de Referência Estratégico Nacional (QREN, Portugal

Antunes, Filipa

Baptista, Inês

Castro, Paula M. L.

Jorge, Ruben

Moreira, Irina S.

Novo, Ana

Saraiva, Isabel

English

Repositório Institucional da Universidade Católica Portuguesa

* Corresponding author: anovo@porto.ucp.pt  Studies on Hydrogen Sulfide Oxidase from Pseudomonas sp.  Ana Novo1*, Filipa Antunes2, Inês Baptista2, Ruben Jorge2, Isabel Saraiva3, Irina S. Moreira1, Paula Maria Lima Castro1  1 CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital – Apartado 2511, 4202-401 Porto, Portugal.  2 WeDoTech - Companhia de Ideias e Tecnologias Lda, CiDEB/ESB, Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital – Apartado 2511, 4202-401 Porto, Portugal.  3 EFACEC – Engenharia e Sistemas, S.A., R. Engº Frederico Ulrich - Apartado 3003, 4471-907 Maia, Portugal   Keywords:  Hydrogen sulfide; biological oxidation, Pseudomonas sp., sulfide oxidase   Background: Biogas resultant from the anaerobic treatment of sludge is composed mainly of CH4 and CO2. H2S, a minor component, is of particular concern due to its corrosive and environmentally hazardous properties. Some microorganisms are able to perform enzymatic oxidation of H2S to sulfur and/or sulfate. Biological treatment using these microorganisms can be an alternative to physical/chemical processes, avoiding the production of secondary waste. Objectives: This work aims at the characterization of enzyme(s) responsible for H2S oxidation in Pseudomonas sp. Methods: Isolates A9, B9 and C1, identified as Pseudomonas spp., were isolated through enrichment from wastewater treatment plant deodorant bioreactor supplied with H2S streams. These strains were screened for the presence of genes responsible for H2S oxidation by PCR using degenerated primers for group II sulfide quinone:oxidoreductase1. Fragments with the expected size were purified from the gel and subjected to sequencing. Translated sequences were compared using the BLAST software. Total protein profiles were screened by SDS-PAGE, before and after the addition of H2S. Activity tests were conducted using crude cell extract as catalyst and measuring the formation of sulfate as the reaction product. Conclusions: The amplified fragment from isolate A9 showed 51% identity with an oxidoreductase from Pseudomonas sp. M1, indicating that this bacterium has the machinery required to the desired activity. The protein profile of the strains when grown in the presence H2S differ from the profile of the same strain grown without sulfide.  References : 1 Pham et al (2008) Microbiology 154:3112-3121.     The authors hereby acknowledge the Agency of Innovation (Agência de Inovação, ADI, Portugal) and Quadro de Referência Estratégico Nacional (QREN, Portugal) which through the project “HbioS-Inovações biotecnológicas na remoção de H2S presente no biogás” (QREN-ADI 21592) provided funding for the realization of this work and to the project PEst-OE/EQB/LA0016/2013. 

Studies on Hydrogen Sulfide Oxidase from Pseudomonas sp.

https://repositorio.ucp.pt/bitstream/10400.14/18512/1/FEMS2015_Abstract_%20Ana%20Novo.pdf

Studies on Hydrogen Sulfide Oxidase from Pseudomonas sp.

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Repositório Institucional da Universidade Católica Portuguesa