Calhau, Conceição

Coelho, Marta

Costa, Eduardo

Costa, Maria do Rosário

Faria, Ana

Norberto, Sónia

Pereira, Miguel

Pintado, M. E.

Silva, Sara

English

Phenolic compounds are widespread in plant-derived foodstuffs and therefore abundant in our diet. There are evidences regarding the positive association of their intake with several diseases prevention. Blueberry leaves are of particular interest as they have been described as being rich in phenolic compounds such as chlorogenic acid and quercetin glicosides. Bioavailability is a major issue regarding the biological impact of these compounds in vivo and remains unclear, with few data available on this matter. Studies in CaCo-2 cells (entrocyte cell line) tested the transport of blueberry leaf infusion (optimized to yield the highest amount total phenolics) across this cell line for different incubation times. From the different compounds originally identified only rutin (9%), quercetin-3- glucoside (Q3glu), chlorogenic (7%) and neochlorogenic acids (23%) were transported across the epithelia cells, after 60, 90 and 120 min. From these compounds, neochlorogenic acid and Q3glu exhibit the highest transport rates (23 and 22%, respectively of the original concentration) while for rutin and chlorogenic acid the transport was lower than 10% of the original amount of compound. It was interesting to note that two metabolites, that were not originally present, caffeic and p-coumaric acids were detected after 30 and 60 min, respectively. From this work it was possible to conclude that neocholorogenic acid and Q3glu are more efficiently transported through the CaCo-2 membrane and that this process resulted in the detection of some metabolites that were not originally present

Repositório Institucional da Universidade Católica Portuguesa

 1                   PORTUGUESE CONGRESS OF  MICROBIOLOGY AND BIOTECHNOLOGY Micro Biotec‘13 6th – 8th December | Aveiro Portugal Abstracts Book  48   Food Microbiology & Biotechnology P017 BIOAVAILABILITY OF PHENOLIC COMPOUNDS FROM BLUEBERRY LEAF INFUSIONS (VACCINIUM CORYMBOSUM L.) IN CACO-2 CELLS  Sara Silva1; Sónia Norberto2; Eduardo Costa1; Miguel Pereira1; Maria do Rosário Costa1; Marta Coelho1; Ana Faria3; Maria Manuela Pintado1; Conceição Calhau4  1CBQF- Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal;  2Departamento de Bioquímica (U38-FCT), Faculdade de Medicina, Universidade do Porto, Centro de Investigação Médica, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal; 3Centro de Investigação em Química (CIQ), Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal & Departamento de Bioquímica (U38-FCT), Faculdade de Medicina, Universidade do Porto, Centro de Investigação Médica, Al.;  4CINTESIS - Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal & Departamento de Bioquímica (U38-FCT), Faculdade de Medicina, Universidade do Porto, Centro de Investigação Médica, A  Phenolic compounds are widespread in plant-derived foodstuffs and therefore abundant in our diet. There are evidences regarding the positive association of their intake with several diseases prevention. Blueberry leaves are of particular interest as they have been described as being rich in phenolic compounds such as chlorogenic acid and quercetin glicosides. Bioavailability is a major issue regarding the biological impact of these compounds in vivo and remains unclear, with few data available on this matter. Studies in CaCo-2 cells (entrocyte cell line) tested the transport of blueberry leaf infusion (optimized to yield the highest amount total phenolics) across this cell line for different incubation times. From the different compounds originally identified only rutin (9%), quercetin-3-glucoside (Q3glu), chlorogenic (7%) and neochlorogenic acids (23%) were transported across the epithelia cells, after 60, 90 and 120 min. From these compounds, neochlorogenic acid and Q3glu exhibit the highest transport rates (23 and 22%, respectively of the original concentration) while for rutin and chlorogenic acid the transport was lower than 10% of the original amount of compound. It was interesting to note that two metabolites, that were not originally present, caffeic and p-coumaric acids were detected after 30 and 60 min, respectively. From this work it was possible to conclude that neocholorogenic acid and Q3glu are more efficiently transported through the CaCo-2 membrane and that this process resulted in the detection of some metabolites that were not originally present.   

Bioavailability of phenolic compounds from blueberry leaf infusions (Vaccinium Corymbosum L.) In caco-2 cells

https://repositorio.ucp.pt/bitstream/10400.14/14349/1/Bioavailability%20of%20phenolic%20compounds%20from%20blueberry%20leaf%20infusions%20%28vaccinium%20corymbosum%20l.%29%20In%20caco-2%20cells.PDF

Bioavailability of phenolic compounds from blueberry leaf infusions (Vaccinium Corymbosum L.) In caco-2 cells

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Repositório Institucional da Universidade Católica Portuguesa