Castro, Paula M. L.

Moreira, Irina S.

Ribeiro, Ana R.

Tiritan, Maria E.

English

Repositório Institucional da Universidade Católica Portuguesa

ENANTIOSELECTIVE BIODEGRADATION OF FLUOXETINE BY THE WILD STRAIN LABRYS PORTUCALENSIS F11  Irina S Moreira1, Ana R Ribeiro123, Maria E Tiritan23 and Paula ML Castro1 1 Centro de Biotecnologia e Química Fina (CBQF), Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal 2 Centro de Química Medicinal da Universidade do Porto (CEQUIMED-UP), Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4050-047 Porto, Portugal 3 Centro de Investigacão em Ciências da Saúde (CICS), Instituto Superior de Ciências da Saúde-Norte, CESPU, Rua Central de Gandra, 1317, 4585-116 Gandra PRD, Portugal ismoreira@porto.ucp.pt  Fluoxetine (FX) is a chiral fluorinated pharmaceutical indicated mainly for treatment of depression and is one of the most dispensed drugs in the world. Environmental contamination with this drug and its active metabolite norfluoxetine (Nor-FX) has been detected in sewage effluents and in surface waters. Microbial degradation of this compound is largely unknown. Moreover, the biodegradation of enantiomers in chiral pharmaceuticals in the environment is scarcely studied.  The biodegradation of FX was achieved by the wild strain Labrys portucalensis F11. When FX was supplied as sole carbon source, strain F11 was able to biodegrade 2 µM of FX in 20 days with stoichiometric liberation of fluoride. When sodium acetate was added, strain F11 was able to biodegrade the total amount of 21 µM of FX, with liberation of half of the stoichiometrically expected fluoride. In both experiments, Nor-FX was detected as intermediary metabolite during biodegradation. Experiments revealed higher biodegradation extent for the R-enantiomeric form of FX and the formation of only one enantiomer of the metabolite Nor-FX as indicated by the enantioselective HPLC method developed. To the best of our knowledge this is the first time that biodegradation of fluoxetine is reported.  Acknowledgements: Irina S. Moreira and Ana R. Ribeiro wish to acknowledge a research grant from Fundação para a Ciência e Tecnologia (FCT), Portugal (Ref. SFRH/BD/28744/2006 and SFRH/BD/64999/2009, respectively) and Fundo Social Europeu (FSE) (Programa Operacional Potencial Humano (POPH), Quadro de Referência Estratégico Nacional (QREN)). This work was supported by the FCT Project - PTDC/EBB-EBI/111699/2009. 

Enantioselective biodegradation of fluoxetine by the wild strain Labrys portucalensis F11

https://repositorio.ucp.pt/bitstream/10400.14/10288/1/Abstract.pdf

Enantioselective biodegradation of fluoxetine by the wild strain Labrys portucalensis F11

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Repositório Institucional da Universidade Católica Portuguesa