Franco, Albina R.

Rodrigues, Rui

Vasconcelos, Marta W.

English

Repositório Institucional da Universidade Católica Portuguesa

Resistance induction to pine wilt disease in four month old seedlings of Pinus spp. by inoculation with an avirulent nematode strain: case study Albina R. Franco, Rui Rodrigues, Marta W. Vasconcelos Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal  Pine wilt disease, caused by a pine wood nematode (PWN) Bursaphelenchus xylophilus (Steiner and Buhrer) Niclke, is seriously infecting Pinus pinaster trees. PWN infections are detected once external symptoms are observed (i.e. chlorosis of the needles), but pine trees could be asymptomatic and serve as a long-term host. Recent studies have demonstrated that tree resistance may be induced by inoculating seedlings with avirulent PWN strains. Although the development of disease and host response has been determined, the resistance mechanisms and plant expression remains unknown. Moreover, it is not known if this avirulent nematode, which has proved to be innocuous for Japanese red pine and black pine, is also avirulent with other Pinus species, in particular Pinus pinaster. The primary goal of this work was to assess the effect of the inoculation of an avirulent isolate of B. xylophilus (C14-5) on different Pinus spp. seedlings (P. sylvestris, P. nigra, P. pinea and P. pinaster). In parallel, seedlings were also inoculated with a virulent strain to compare the effects between the two strains of nematodes. The resistance mechanisms of PWN were assessed during the first stages of infection. The effect of seedling inoculation was determined in terms of the expression of several Pinus genes potentially involved in the disease response. Finally, PWN progression was also monitored to confirm successful seedling inoculation. 

Resistance induction to pine wilt disease in four month old seedlings of Pinus spp. by inoculation with an avirulent nematode strain: case study

https://repositorio.ucp.pt/bitstream/10400.14/5845/1/trab-int_2009_ESB_1620_Vasconcelos_Marta_01.pdf

Resistance induction to pine wilt disease in four month old seedlings of Pinus spp. by inoculation with an avirulent nematode strain: case study

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Repositório Institucional da Universidade Católica Portuguesa