Andrade, J.C.

Gonzalez-Paluelo, M.

Vasconcelos, I.

English

Repositório Institucional da Universidade Católica Portuguesa

IV Iberian Congress on BiotechnologyI Ibero-American Meeting on BiotechnologyBIOTEC'98BOOK OF ABSTRACTSEdited byMANUEL MOTA and EUGENIO C. FERREIRABIOTEC'98	 BBE060Fermentation of Low Grade Glycerol by Clostridium butyricum andClostridum acetobutyllcum StrainsM. Gonzalez-Paluelo, J.C. Andrade and I. VasconcelosEscola Superior de Biotecnologia, Porto PORTUGALKeywords: 0103104 1,3-propanediol butanol, CI. butyricum. Cl. acetobutylicumIn recent years there has been an increasing interest in using Biodiesel as a less pollutant, alternativefuel I I h The Biodiesel is produced by the transesterification of plant seed oils and yields glycerol asthe main by-product. A subsequent utilisation of this "low grade" glycerol could help to decreasethe Biodiesel production costs and facilitate the implementation of Biodiesel as an alternative fuel.It has been shown that some clostridial species can ferment glycerol. Clostridium butyricum canuse glycerol as the sole carbon source with a high conversion to 1,3-propanediol, which can beused as a monomer on biodegradable polymers synthesis. Clostridum acetobutylicum can useglycerol as a co-substrate of glucose to produce solvents such as butanol.In this work a low grade glycerol, obtained through the transesterification process using rape seedoil, was used without prior purification.Batch fermentations regulated at pH 6.5 were performed with CL butyricum VPI 3266 and. VPI1718. A concentration of glycerol as high as 40 g/I could be fermented with a maximumconversion of 0.60 g 1,3-pmpanediol/ g glycerol consumed and a 100% consumption of thisglycerol.Continuous cultures of CL acetobutylicum ATCC 4259 were performed at different pH values.A maximum concentration of butanol of 8.4 g/I was achieved.Attempts to optimize culture conditions in order to improve the efficiency of these processes arestill under investigation.(II Chowdhury,J and Fouhy, K, Chem. Eng., 100, 35-39, 1993.260

Fermentation of Low Grade Glycerol by Clostridium butyricum and Clostridum acetobutyllcum Strains

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Fermentation of Low Grade Glycerol by Clostridium butyricum and Clostridum acetobutyllcum Strains

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Repositório Institucional da Universidade Católica Portuguesa