Alvarez Alvarez, José María

Botet Vaca, Ramón

Garcés Claver, Ana

citaREA Repositorio Electrónico Agroalimentario

  Identification of QTLs for Fom race 1.2 resistance using a genotyping by sequencing approach  Ramon Botet 1,2, Ana Garcés-Claver1, José Mª Álvarez1   1Agrifood Research and Technology Centre (CITA). Avda. Montañana, 930. 50059, Zaragoza, Spain. 2Laboratory of Genetics, Wageningen University and Research (WUR), P.O. Box 16,  6700 AA, Wageningen, The Netherlands Corresponding author. E-mail: agarces@cita-aragon.es. Tfno: +34976716560. Fax: +34976716335  Fusarium wilt of melon (Cucumis melon L.), caused by Fusarium oxysporum Schlecht. f. sp. melonis Snyd. & Hans. (Fom) is an important disease spread worldwide. Race 1.2, subdivided into pathotype 1.2y (Yellow) and 1.2w (Wilt), overcomes all resistance genes described and could become a serious threat to this crop cultivation. The development of cultivars exhibiting enhanced resistance to Fom race 1.2 is an important objective of breeding programs. Partial resistance to Fom race 1.2 was studied using a recombinant inbred line (RIL) population derived from a cross between cv. ‘Piel de Sapo’, a highly valuable cultivar, and ‘BG-5384’, a partially resistant line. Artificial inoculations were performed with a yellow strain and a wilt one and two environments, growth chamber and greenhouse. Genotyping was carried out by using genotyping-by-sequencing approach. Genotypic data for 2625 single nucleotide polymorphism (SNP) markers on twelve chromosomes were used for the association analysis, revealing polygenic control with 10 different small effect QTLs. Those showed to be environment specific, probably due to a high RIL –environment interaction, and pathotype specific in most cases. These results match with previously reported research. Further work will be made on fine-mapping regions of interest and determining the molecular basis of such a complex resistance.   Key words: disease resistence, genotyping by sequencing, melon, RILs, QTLs.  

Identification of QTLs for Fom race 1.2 resistance using a genotyping by sequencing approach: póster

http://citarea.cita-aragon.es/citarea/bitstream/10532/2968/2/2015_160ab.pdf

Identification of QTLs for Fom race 1.2 resistance using a genotyping by sequencing approach: póster

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