Antunes, Luis Eduardo Corrêa

Diniz, Angêla C.

Fachinello, José Carlos

Hass, Luciane B.

Marafon, Anderson C.

Pereira, Iván S.

Pina Sobrino, Ana

Prezotto, Marcos E.

citaREA Repositorio Electrónico Agroalimentario

International Symposium on Vegetable Grafting 3 – 5 Oct. 2011, Viterbo - Italy 112  [P71] Graft compatibility in peach: growth analysis and phenylalanine ammonia-lyase expression  Ivan S. Pereira1*,  Anderson C. Marafon2, Angêla. C. Diniz3, Luis E. C. Antunes3, Marcos E. Prezotto1, Luciane B. HAAS1, José C. Fachinello1, Ana Pina4  1Departamento de Fruticultura, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil 2Sistemas de Produção Sustentáveis, Embrapa Tabuleiros Costeiros (CPATC), Rio Largo, Brazil 3Departamento de Fruticultura, Embrapa Clima Temperado (CPACT), Pelotas, Brazil 4Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Zaragoza, Spain *Email: ivanspereira@gmail.com  Keywords: FCC, graft incompatibility, PAL activity, Prunus, vegetative growth  The phenylalanine ammonia-lyase (PAL) is considered a key enzyme of the phenylpropanoid pathway. This pathway is responsible for biosynthesis of many secondary compounds, such as anthocyanins, flavanols and lignins. The higher level of PAL is associated with accumulation of phenolic compounds in the union of the incompatible grafts. The aim of this work was to identify incompatibles combinations according to tree vegetative growth and to determine the PAL expression and activity in the bark as a biochemical indicator of graft incompatibility in Prunus. The study was performed two years after grafting with three rootstocks: ‘Capdeboscq’ (Prunus persica L. Batsch), ‘Tsukuba 1’ (P. persica L. Batsch) and ‘Umezeiro’ (P. mume Sieb. et Zucc.) grafted into the scion ‘Chimarrita’ (P. persica L. Batsch). The vegetative characteristics were measured and the results indicated that ‘Umezeiro’ rootstock induces a feeble growth of the scion resulting in the death of some trees. However, the others rootstocks tested showed a vigorous growth without dead trees through all years of evaluation. The PAL expression and activity was higher in ‘Umezeiro’ than in the other combinations. In conclusion, our data show that ‘Umezeiro’ presents high level of incompatibility with ‘Chimarrita’ and that the PAL gene expression and activity can be used as biochemical indicators of graft incompatibility.      

Graft compatibility in peach: growth analysis and phenylalanine ammonia-lyase expression

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Graft compatibility in peach: growth analysis and phenylalanine ammonia-lyase expression

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citaREA Repositorio Electrónico Agroalimentario