Alabart Alvarez, José Luis

Cocero Oviedo, Mª Jesús

Echegoyen Pérez, Elías

Folch Pera, José

Lahoz Crespo, Belén

Sánchez Ruiz, Pilar

citaREA Repositorio Electrónico Agroalimentario

26th Annual Meeting  A.E.T.E. – Kuopio, Finland, 10th -11th September 2010 i 26ème  COLLOQUE  SCIENTIFIQUE _________________________________  26th SCIENTIFIC  MEETING * *     * Dr Yvan HEYMAN  Special Celebration *     * * ____________________________________________________ KUOPIO, Finland, 10th and 11th September 2010 ____________________________________________________ 26th Annual Meeting  A.E.T.E. – Kuopio, Finland, 10th -11th September 2010 124 EFFECT OF A DEFINED OR AN UNDEFINED IN VITRO MATURATION MEDIUM ON CLEAVAGE RATE AND EMBRYO DEVELOPMENT IN SHEEP  ALABART JL1, LAHOZ B1, FOLCH J1, SÁNCHEZ P1, ECHEGOYEN E1,  COCERO MJ2  1Centro de Investigación y Tecnología Agroalimentaria (CITA) de Aragón, Zaragoza, Spain;  2Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA),  Madrid, Spain.  Ovine oocytes matured in vitro are compromised in their developmental capacity compared with in vivo matured counterparts. While the causes of these differences are not fully elucidated, it is known that in vitro maturation (IVM) can be influenced by culture media composition and culture conditions used for IVM as well as by variations in oocyte quality. Follicular fluid (FF) is a natural medium of nuclear and cytoplasmic maturation of mammalian oocytes in vivo, suggesting that it may be suitable for oocyte culture. Epidermal growth factor (EGF) and follicle stimulating hormone (FSH) are also known to play a key role in nuclear and cytoplasmic maturation, and cysteamine (Cys) enhances glutathione synthesis, which is known to protect cells from oxidative damage. The aim of the present study was to compare the efficiency of a defined IVM medium (TCM199 supplemented with 100 µM Cys and 10 ng/ml EGF; Cys+EGF) with an undefined medium (TCM199 supplemented with 132 µg/ml pFSH and 10% FF; FSH+FF) for in vitro production (IVP) of ovine embryos, using abattoir-derived oocytes (170 COCs) from Rasa Aragonesa ewes. Cleavage rates were 74.3% (52/70) and 80.0% (80/100) for Cys+EGF and FSH+FF groups, respectively (fresh semen; 2·106 spermatozoa/ml). Blastocyst rates at day 8 were 40.4% in Cys+EGF and 33.7% in FSH-FF groups, with an increase of 5.8 and 3.7%, respectively, when compared with blastocyst rates at day 7. Final yield of embryo IVP was 30.0% (21/70) when Cys+EGF medium was used for IVM, and 27.0% (27/100) when using FSH+FF medium. Day 7 and 8 good morphological quality blastocysts were vitrified, and some of them were transferred to synchronized recipients, reaching 9.1% (1/11) and 10.0% (1/10) lambs born in Cys+EGF and FSH+FF groups, respectively. Differences were not significant between groups for any of the studied variables.  Under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient, defined, maturation medium for IVP of embryos from abattoir-derived oocytes that provides an efficiency equivalent to that achieved using supplementation with FF and FSH. Further studies are needed to elucidate the role of different factors in IVM in sheep and to check the efficacy of this defined medium with oocytes from different sources.  

Effect of a defined or an undefined in vitro maturation medium on cleavage rate and embryo development in sheep

http://citarea.cita-aragon.es/citarea/bitstream/10532/1753/1/2011_398.pdf

Effect of a defined or an undefined in vitro maturation medium on cleavage rate and embryo development in sheep

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citaREA Repositorio Electrónico Agroalimentario