Alabart Alvarez, José Luis

Calvo Lacosta, Jorge Hugo

Folch Pera, José

Lahoz Crespo, Belén

Martínez Royo, Alberto

citaREA Repositorio Electrónico Agroalimentario

EAAP – 62nd Annual Meeting, Stavanger 2011 189Detection of QTL affecting serum cholesterol, LDL, HDL, and triglyceride in pigsCinar, M.U., Uddin, M.J., Duy, D.N., Tesfaye, D., Tholen, E., Juengst, H., Looft, C. and Schellander, K., Institute of Animal Science, University of Bonn, Animal Breeding and Husbandry, Endenicher Allee 15, 53115 Bonn, Germany; ucin@itw.uni-bonn.deThe objective of this research was to investigate the chromosomal regions influencing the serum level of the total cholesterol (CT), triglyceride (TG), high density protein cholesterol (HDL) and low density protein cholesterol (LDL) in pigs. For this purpose, a total of 330 animals of the Duroc × Pietrain F2 population were phenotyped for serum lipids using ELISA and were genotyped by using 122 microsatellite markers covering all porcine autosomes for QTL study in QTL Express. Blood sampling were performed at approximately 175 days just before slaughter of the pig. Most of the traits were correlated with each other and were influenced by average daily weight gain, slaughter date and age at slaughter of the animals. A total of 18 QTL including three QTL with imprinting effect were identified on 11 different porcine autosomes. Most of the QTL reached to 5% chromosome-wide (CW) level significance including a QTL at 5% experiment-wide (GW) and a QTL at 1% GW level significance. Of these QTL, four were identified for both the CT and LDL, whereas two QTL were identified for both the TG and LDL. Moreover, three chromosomal regions were detected for the HDL/LDL ratio in this study. A QTL for HDL on SSC2 and two QTL for TG on SSC11 and 17 were detected with imprinting effect. The highly significant QTL (1% GW) was detected for LDL at 82 cM on SSC1, whereas significant QTL (5% GW) was identified for HDL/LDL on SSC1 at 87 cM. Chromosomal regions with pleiotropic effects were detected for correlated traits on SSC1, 7 and 12. Two novel QTL on SSC16 for HDL and HDL/LDL ratio and an imprinted QTL on SSS17 for TG were detected in the pig for the first time. The results of this work shed additional light on the genetic background of serum lipid concentrations and these findings will be helpful to identify candidate genes in these QTL regions related to lipid metabolism and serum lipid concentrations in pigs.Characterization of the gene Melatonin Receptor 1A (MTNR1A) in the Rasa Aragonesa sheep breed: association with reproductive seasonality Martinez-Royo, A.1, Lahoz, B.1, Alabart, J.L.1, Folch, J.1 and Calvo, J.H.2, 1CITA, Producción Animal, Av Montañana 930, 59059 Zaragoza, Spain, 2ARAID, Fundación, 50004 Zaragoza, Spain; amartinezroyo@aragon.esThis work focuses on the characterization and evaluation of MTNR1A as a candidate gene related to reproductive seasonality in the Rasa Aragonesa sheep breed. MTNR1A has shown influence on reproductive seasonality in other breeds. Cyclic and non cyclic ewes for a given month were classified as a dichotomous variable coded with ‘1’ and ‘0’, respectively. The percentage of cyclic ewes between February and July 2009 was analyzed using a generalized linear model for categorical variables with repeated measures by the CATMOD procedure of SAS. The model used was FC(ik) = μ + gene(i) + month(k) + GxM(ik), where FC(ik) is the frequency of cyclic ewes with genotype(i) and month (k); μ is the overall mean percentage of cyclic ewes from the total set; gene(i) is the effect of genotype(i) nested to the ram; month (k) is the effect of the month, treated as a dichotomous repeated variable cyclic/non cyclic within 6 months of the experimental tests; GxM (ik) is the interaction effect. A significant effect was found between SNP606 of the MTNR1A gene and spontaneous out of season estrus behaviour. The T allele was associated with cyclicity in the Rasa Aragonesa breed. This finding, along with the fact that this polymorphism does not result in an amino-acid substitution, suggest that SNP606 may act in linkage equilibrium with a mutation in other genes responsible for out of season breeding. New polymorphisms (11 SNPs) in the coding region of the gene MTNR1A did not show any association for reproductive cyclicity during anestrus in the Rasa Aragonesa breed. Association between polymorphisms (17 SNPs) found in the promoter region and cyclicity is expected to be completed soon.Session 30 Poster 33Session 30 Poster 32

Characterization of the gene Melatonin Receptor 1A (MTNR1A) in the Rasa Aragonesa sheep breed: association with reproductive seasonality

http://citarea.cita-aragon.es/citarea/bitstream/10532/1694/1/2011_306.pdf

Characterization of the gene Melatonin Receptor 1A (MTNR1A) in the Rasa Aragonesa sheep breed: association with reproductive seasonality

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citaREA Repositorio Electrónico Agroalimentario