Garcés Claver, Ana

González Pérez, Susana

Mallor Giménez, Cristina

Merino, Fuencisla

Pomar, Federico

Rivera, Antonio

Silvar, Cristina

Taboada, Alfredo

citaREA Repositorio Electrónico Agroalimentario

__________________________________ Session 8: Evolution and Biodiversity409S8/P3: GENETIC CHARACTERIZATION AND PUNGENCYANALYSIS IN A COLLECTION OF ONION LANDRACES FROMNW SPAINSusana González-Pérez1, Cristina Mallor2, Ana Garcés-Claver2, FuencislaMerino1, Alfredo Taboada3, Antonio Rivera3, Federico Pomar1, Cristina Silvar11Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, E-15071,A Coruña, Spain susana.gperez@udc.es2Centro de Investigación y Tecnología Agroalimentaria de Aragón, Avd. Montañana930, E-50059, Zaragoza, Spain3Centro de Investigaciones Agrarias de Mabegondo, Ctra. Betanzos-Santiago km 7.5,E-15318, A Coruña, SpainOnions (Allium cepa L.) are one of the oldest cultivated vegetables all around theworld, where they are considered as the second most valuable horticultural crop,following only tomato. Spain is the second producer of onion in Europe with a growingarea of ca. 25,000 ha. About the 5 % of this surface is located in Galicia (NW Spain),where this crop represents the fourth most important vegetable according to cultivatedarea and production (www.magrama.gob.es). In Galicia, onions are mainly present as acollection of distinctive landraces, tradicionally preserved by local growers due to theirhigh quality and good acceptance in local markets.Twenty local onion varieties were collected in 1998 at the main productive regions inNW Spain and they are kept at the CIAM (Centro de Investigaciones Agrarias deMabegondo) germplasm bank. This collection of onions was previously evaluated foragro-morphological traits, showing excellent organoleptic qualities (Rivera-Martínez etal. 2005). Such results point to these Galician landraces as a potentially valuablereservoir of interesting traits which have not been fully exploited and that might highlycontribute to increase the primary genepool of current onion cultivars. An evaluation ofthe diversity represented by this genetic resource is necessary in order to facilitate itsuse in future cultivar development by breeders.In the current work, eighteen of these lines were evaluated with a set of seventeenmicrosatellite markers previously described in the literature. Analysis of pungency,based on the pyruvic acid content, was also assayed. Genetic data revealed a goodlevel of polymorphism among tested markers, with PIC (Polymorphic InformationContent) values ranging from 0.29 to 0.69 and a mean number of alleles per locus ofthree. The onions collection showed a wide range of heterozigosity, with all genotypesexhibiting at least one heterozigous locus. Cluster analysis grouped all lines with asimilarity of 0.55. Preliminary observations suggest that landraces are groupedirrespective of their local origin and that clustering pattern is more on the basis ofmorphological traits. Analysis of pyruvic acid also point to a high frequency ofheterozygosity whithin the lines analyzed. Considering the mean values obtained fromten independent bulbs, five onion landraces could be classified as “extra-sweet”, fouras “sweet”, seven “of moderate pungency” and two as “pungent”.Rivera-Martínez et al. (2005). Spanish J Agric Res, 3(1): 90-97Acknowledgments: this work was funded by MICINN-INIA-FEDER (project RTA2011-00118)

Genetic characterization and pungency analysis in a collection of onion landraces from NW Spain: abstract

http://citarea.cita-aragon.es/citarea/bitstream/10532/2347/1/2013_183.pdf

Genetic characterization and pungency analysis in a collection of onion landraces from NW Spain: abstract

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citaREA Repositorio Electrónico Agroalimentario