低温オーブントラッピングキャピラリーガスクロマトグラフィーによるヒト全血中アセトニトリル及びプロピオニトリルの高感度分析

Abstract

We have developed a simple and sensitive method for determining acetonitrile and propionitrile in human whole blood using capillary gas chromatography (GC) with cryogenic oven trapping. After heating 0.5 ml of a whole blood sample containing both nitriles and 0.5 ml of distilled water in a 7-ml vial at 75℃ for 15 min, 5 ml of the headspace vapor was drawn into a 10-ml syringe and injected to GC with a flame thermionic detector. A sharp peak was obtained for acetonitrile at -10℃ of the initial oven temperature. The extraction efficiencies for acetonitrile and propionitrile were 0.27 and 0.40%, respectively. For quantitation of acetonitrile, propionitrile was used as internal standard, and vise versa. The calibration curves for both compounds gave good linearity in the range of 0.2-6 μg/ml whole blood. The detection limits (signal to noise ratio = 3) were 0.05μg/ml for acetonitrile and 0.01μg/ml for propionitrile. The coefficients of intra-day variations were 1.0% at 0.4μg/ml and 1.7% at 2μg/ml for acetonitrile; 7.1% at 0.4μg/m1 and 1.0 % at 2μg/ml for propionitrile. This method can be applicable for determining these nitriles in whole blood samples in acute and chronic intoxication cases.rights:日本法中毒学会rights:本文データは日本法中毒学会の許諾のもと掲載しています