Akihito, Yokoyama

Atsuya, Nobumoto

Chie,  Nishioka

Jing, Yang

Keiko, Udaka

Masayuki, Tsuda

Mutsuo, Furihata

Satoshi, Serada

Takayuki, Ikezoe

Tetsuji, Naka

Kochi University Repository

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27.CD34+/CD38- acute myelogenous leukemia cells aberrantly express CD82 adhesion molecule. 
Chie Nishioka1), 6) , Takayuki Ikezoe2), Mutsuo Furihata3), Jing Yang2), Satoshi Serada4), Tetsuji Naka4), Atsuya 
Nobumoto5), Masayuki Tsuda5), Keiko Udaka1) and Akihito Yokoyama 2) 
1) Department of immunology, 2) Hematology and Respiratory Medicine, 3)Tumor Pathology,  
5) The Facility for Animal Research, Kochi Medical School,  
4) Laboratory for immune Signal, National Institute of Biomedical Innovation,  
6) Research Fellow of the Japanese Society for the Promotion of Science (JSPS)  
To identify molecular targets in leukemia stem cells (LSCs), this study compared the protein expression profile of 
freshly isolated CD34+/CD38- cells with that of CD34+/CD38+ counterparts from individuals with acute 
myelogenous leukemia (n=2, AML) using isobaric tags for relative and absolute quantitation (iTRAQ). A total of 
98 proteins were overexpressed, while six proteins were underexpressed in CD34+/CD38- AML cells compared 
with their CD34+/CD38+ counterparts. Proteins overexpressed in CD34+/CD38- AML cells included a number of 
proteins involved in DNA repair, cell cycle arrest, gland differentiation, anti-apoptosis, adhesion, and drug 
resistance. Aberrant expression of CD82, a family of adhesion molecules, in CD34+/CD38- AML cells was noted 
in additional clinical samples (n=12) by flow cytometry. Importantly, down-regulation of CD82 in CD34+/CD38-
AML cells by a short hairpin RNA (shRNA) stimulated their migration via up-regulation of matrix 
metalloproteinases 9 (MMP9), as assessed by transwell assay and real time RT-PCR, respectively. Moreover, we 
found that down-regulation of CD82 in CD34+/CD38- AML cells by an shRNA significantly impaired 
engraftment of these cells in severely immunocompromised mice. Taken together, aberrant expression of CD82 
might play a role in adhesion of LSCs to bone marrow microenvironment. CD82 could be an attractive molecule 
target to eradicate LSCs. 


CD34+/CD38- acute myelogenous leukemia cells aberrantly express CD82 adhesion molecule.

CD34+/CD38- acute myelogenous leukemia cells aberrantly express CD82 adhesion molecule.

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