The most likely determinants are mutations in the nonstructural proteins encoding viral replication and protein cleavage mechanisms

Bakonyi

Beasley

Bondre

Borisevich

Burt

Campbell

Castle

Coia

Davis

Ding

Elizabeth M. Botha

Gustavio Palacios

Hurrelbrink

Janusz T. Paweska

Keller

Kinney

Kumar

Lanciotti

Lindenbach

Louis H. Nel

Mariaan Wolfaardt

Marietjie Venter

Petersen

Puig-Basagoiti

Robert Swanepoel

Shurtleff

Smithburn

Stocks

Thompson

Venter

Von Heijne

Wanda Markotter

Wicker

Yamshchikov

Emerging Infectious Diseases

English

PubMed

We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3´ noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study.This study was funded by the National Research Foundation, South Afric

Botha, Elizabeth Magdelena

Markotter, Wanda

Wolfaardt, Marianne

Paweska, Janusz Tadeusz

Swanepoel, Robert

Palacios, Gustavo

Nel, Louis Hendrik

Venter, Marietjie

UPSpace at the University of Pretoria

Genetic determinants of virulence in pathogenic lineage 2 West Nile virus strains

We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3′ noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study

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Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains

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Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains

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