Harding Passey melanoma in the BALB/C mouse as a model for studying the interactions between host macrophages and tumor cells

Abstract

Transplants and primary cultures of the Harding Passey Melanoma (HPM) were found to consist of melanocytes and host macrophages. A pure population of melanocytes (obtained by subculturing) produced tumors when ip injected into Balb/c mice. There was a progressive infiltration of the tumors by peritoneal macrophages (PM). Repeated inoculations of irradiated HPM cells ip induced in vivo a protection against HPM graft. In vitro spleen cells produced a nonspecific inhibition of thymidine uptake by HPM. Normal PM were found to exhibit a spontaneous nonspecific inhibition. This capacity was impaired in PM taken from immune animals 7 days after immunization and recovered 31 days after. The relevance of such findings to cell mediated immunity and lysosome function are discussed.SCOPUS: ar.jinfo:eu-repo/semantics/publishe