ANDRADE, M. A.

ARNHOLD, E.

DUARTE, S. C.

JAYME, V. de S.

MORAES, D. M. C.

PANIAGO, M. E. L.

Portuguese

Repository Open Access to Scientific Information from Embrapa

Correspondente ao trabalho a ser apresentado em forma de poster no EggMeat 2015  CARACTERIZAÇÃO FENOTÍPICA E DETECÇÃO MOLECULAR DE Salmonella sp. NAS FASES DE CRIA, RECRIA E PRODUÇÃO EM LOTE DE POEDEIRAS COMERCIAIS  PHENOTYPICAL CHARACTERIZATION AND MOLECULAR DETECTION OF Salmonella IN SAMPLES IN STARTER, GROWER AND EGG PRODUCTION PHASES IN COMMERCIAL LAYING HENS  DUNYA MARA CARDOSO MORAES1, SABRINA CASTILHO DUARTE2 MARIA EDUARDA LEMES PANIAGO3, EMMANUEL ARNHOLD4 VALÉRIA DE SÁ JAYME4, MARIA AUXILIADORA ANDRADE4  1 – Médica Veterinária – bolsista CAPES, Brasil 2 – Pesquisadora da EMBRAPA Suínos e Aves, Brasil 3 – Médica Veterinária Autônoma 4 – Professor da Escola de Veterinária e Zootecnia da Universidade Federal de Goiás, Brasil   ABSTRACT: This present study was developed with the objective of detecting Salmonella sp. by conventional bacteriology and real time PCR techniques in samples of transport crate’s flooring material (meconium), of raising environment (swab of cages and drinking fountains), swab of cloaca, food and insects from the growing, laying and production periods in a lot of commercial laying hens. Of the 864 samples were collected, among whom 248 samples originated from growing, 392 from laying and 224 from production. Of the 864 samples, 2,8% where positives in bacteriologic technique and 15,3% in real time PCR. The contamination was higher in the periods of growing and laying and declined in the period of production. Twenty four isolations of Salmonella where typified as Salmonella Agona (41,7%), Salmonella Livingstone (33,3%), Salmonella Cerro (16,7%), Salmonella Senftenberg (4,2%) and Salmonella Schwarzengrund (4,2%). At the period of growing Salmonella Livingstone was identified. This findings suggest vertical contamination in the lot. At the periods of laying and production, isolated material belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude with this study that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in laying period is higher than growing and production periods.  Keywords: Alphitobius diaperinus, environment, cage flooring.      

Phenotypical characterization and molecular detection of salmonella in samples in starter, grower and egg production phases in commercial laying hens.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1037100/1/final7829.pdf

Phenotypical characterization and molecular detection of salmonella in samples in starter, grower and egg production phases in commercial laying hens.

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa