BRASIL, B. dos S. A. F.

BRUNALE, P. P. de M.

FERNANDES, M.

HADI, S. I. I. A.

SANTANA, H.

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[Cod. Trabalho: 121]INDUSTRIAL & ENVIRONMENTAL BIOTECHNOLOGYMicrobial Degradation: Wastes and EffluentsSELECTION OF MICROALGAE FOR GROWTH IN SUGARCANE VINASSEHUGO SANTANA1; PATRÍCIA PORTELA DE MEDEIROS BRUNALE2; MAIARA FERNANDES3; SAMED IBRAHIM ISA ABDEL HADI3;BRUNO BRASIL2.1.UNIVERSIDADE FEDERAL DA BAHIA, VITORIA DA CONQUISTA - BA - BRASIL; 2.EMBRAPA, BRASÍLIA - DF - BRASIL;3.UNIVERSIDADE FEDERAL DO TOCANTINS, GURUPI - TO - BRASIL.Palavras-chave: biofuel;biorefinary;genetic resourcesResumo:Sugarcane vinasse is an ethanol distillation byproduct produced at proportions of 10-15L per liter of ethanol. Due to its low pH and highchemical and biochemical oxygen demands (COD and BOD, respectively) it is considered an environment pollutant and cannot be directlydisposed in aquatic environments. Microalgae are recognized as a promising alternative source for biofuels production and can be grownusing wastewater and carbon emissions from industrial plants. Considering this characteristic, microalgae can be used in a bio refinerystrategy for oil production associated to production of other microalgae-derived products, such as pigments, animal fed and/or fertilizers.This strategy could be applied in an industrial plant using the vinasse and the CO2 produced during the ethanol distillation. Studies focusingin the vinasse pretreatment, through clarification or anaerobic bio digestion, in order to favor microalgae growth have been reported.However, research programs aiming the selection and adaptation of microalgae strains for growth on untreated vinasse are not available. Inhere we show the screening of Brazilian microalgae strains (n=40) deposited in Embrapa´s collection for growth in vinasse. Two parallelstrategies have been used: I) Vinasse gradient agar plates; II) Vinasse liquid media. The incubation conditions were 18h/6h light/dark cyclesat 16.000 Lux at 30 degrees Celsius. In the first approach, unialgae strains were shown in agar plates containing linear gradients of vinasse(0-100%) in BBM or BG11 medium. In the second approach the same strains were grown under concentrations of 25%, 50% and 75%vinasse diluted in distilled water using aerated flasks (5L/h of atmospheric air). In the first approach, one strain was selected, the LBA8, thatwas capable of growing in the entire plate surface. In the second approach, it was possible to select four strains capable of growing at 75%vinasse concentration: LBA39, LBA32, LBA40 and also LBA8. In a first evaluation, all selected strains were identified as green algae(Chlorophyta) species. The selected strains were submitted to cultivation in aerated flasks containing 100% vinasse for 8 days for biomassproductivity measurements. The following measures were taken for each strain: biomass productivity / biomass yield. The results for LBA8were, respectively: 370 mg.d-1.L-1 / 2 2.62 g.L-1; The results for LBA32 were, respectively: 440 mg.d-1.L-1 / 3.10 g.L-1; The results forLBM39 were, respectively: 360 mg.d-1.L-1 / 2.50 g.L-1; the results for LBM40 were, respectively: 360 mg.d-1.L-1 / 2.52 g.L-1. The findingspresented here demonstrate that highly productive microalgae strains can be cultivated in pure untreated vinasse.Itarget Tecnologia - Versão para impressão https://icongresso.itarget.com.br/useradm/usr.nov.trab.view.php?lng=P...1 de 1 24/09/2014 11:43

Selection of microalgae for growth in sugarcane vinasse.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1002372/1/ResumoIBS2014HugoSantana.pdf

Selection of microalgae for growth in sugarcane vinasse.

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