BROWN, G. G.

CERRI, C. C.

DRAKE, H. L.

SCHULZ, K.

TSAI, S. M.

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N-1303Methanogenesis and Associated Prokaryotes in GutContents of the Methane-Emitting Earthworm Eudriluseugeniae from Brazil.K. Schulz1, S. Hunger1, G. G. Brown2, S. M. Tsai3, C. C. Cerri4, H. L. Drake1;1Univ. of Bayreuth, Bayreuth, Germany, 2Embrapa Florestas, Colombo, Brazil,3Univ. of São Paulo, São Paulo, Brazil, 4Univ. of São Paulo, São Paulo, BrazilThe in situ conditions of the earthworm gut provide an ideal transient habitat for ingested anaerobes. Itwas recently discovered that the earthworm Eudrilus eugeniae emits methane. The objective of thisstudy was to resolve the trophic interactions of methanogens and other prokaryotes in gut contents ofE. eugeniae. RNA stable isotope probing (RNA SIP) of bacterial 16S rRNA and mcrA of methanogenswas performed with [13C]-glucose as a model substrate in anoxic microcosms of gut contents. Glucoseconsumption yielded numerous fermentation products, and facultative aerobes (e.g., Aeromonadaceae)and obligate anaerobes (e.g., Lachnospiraceae, Veillonellaceae, and Ruminococcaceae) wereassociated with the diverse fermentations. Methanogenesis was ongoing during incubations but notsignificantly stimulated by supplemental glucose and fermentation products, indicating that endogenousmethanogenic substrates were not limiting relative to methanogenesis. Methanobacteriaceae andMethanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceaeappeared to participate in the complex anaerobic food web. Methanogenesis was significantlystimulated in enrichment cultures by H2-CO2 and methanol but not by formate and acetate. Thesefindings provide insight on the processes and associated taxa potentially linked to the turnover oforganic carbon and methanogenesis in the alimentary canal of E. eugeniae.N-1303. Methanogenesis and Associated Prokaryotes in Gut C... http://www.asmonlineeducation.com/php/asm2014abstracts/dat...1 de 1 13/10/2014 09:19

Methanogenesis and associated prokaryotes in gut contents of the methane-emitting earthworm Eudrilus eugeniae from Brazil.

RCAAP - Repositório Científico de Acesso Aberto de Portugal

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/997979/1/2014GeorgeBGMASMMethanogenesis1.pdf

Methanogenesis and associated prokaryotes in gut contents of the methane-emitting earthworm Eudrilus eugeniae from Brazil.

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