Acute abdomen is a common presentation to many emergency departments worldwide. It is important to differentiate self-limiting causes and serious surgical causes. Inflammatory markers such as WCC and CRP currently being used to determine the cause, assess severity and guide further investigation for acute abdomen. The aim of this study was to determine diagnostic accuracy of C - reactive protein and White Cell Count in abdomino-pelvic diseases of non-traumatic acute abdomen by taking CT scan findings as gold standard. We found that CRP levels of above 100 were highly sensitive (94%) and specific (92%) for an acute surgical abdomen. WCC was not as accurate at determining an acute surgical abdomen due to reasons such as neutropenic sepsis

Ali, D.

Canelo, Ruben

Chauhan, M.N.

Jonker, Leon

Rehman, Shafiq

Riaz, M.

Zaman, S.

Insight - University of Cumbria 

Chauhan, M.N., Rehman, Shafiq, Riaz, M., Jonker, Leon, Ali, D., Zaman, S. and Canelo, Ruben (2018) Diagnostic accuracy of CRP & WCC in abdomino-pelvic diseases of non-traumatic acute abdomen by taking CT findings as gold standard. Austin Journal of Surgery, 5 (5). p. 1142. Downloaded from: http://insight.cumbria.ac.uk/id/eprint/3848/Usage of any items from the University of  Cumbria’s  institutional repository ‘Insight’  must conform to the  following fair usage guidelines.Any item and its associated metadata held in the University of Cumbria’s institutional  repository Insight (unless stated otherwise on the metadata record) may be copied, displayed or performed, and stored in line with the JISC fair dealing guidelines (available here) for educational and not-for-profit activitiesprovided that• the authors, title and full bibliographic details of the item are cited clearly when any partof the work is referred to verbally or in the written form • a hyperlink/URL to the original Insight record of that item is included in any citations of the work• the content is not changed in any way• all files required for usage of the item are kept together with the main item file.You may not• sell any part of an item• refer to any part of an item without citation• amend any item or contextualise it in a way that will impugn the creator’s reputation• remove or alter the copyright statement on an item.The full policy can be found here. Alternatively contact the University of Cumbria Repository Editor by emailing insight@cumbria.ac.uk.Citation: Chauhan MN, Rehman S, Riaz M, Jonker L, Ali D, Zaman S, et al. Diagnostic Accuracy of CRP & WCC in Abdomino-Pelvic Diseases of Non-Traumatic Acute Abdomen by Taking CT Findings as Gold Standard. Austin J Surg. 2018; 5(5): 1142.Austin J Surg - Volume 5 Issue 5 - 2018ISSN : 2381-9030 | www.austinpublishinggroup.com Canelo et al. © All rights are reservedAustin Journal of SurgeryOpen AccessAbstractAcute abdomen is a common presentation to many emergency departments worldwide. It is important to differentiate self- limiting causes and serious surgical causes. Inlammatory markers such as WCC and CRP currently being used to determine the cause, assess severity and guide further investigation for acute abdomen. The aim of this study was to determine diagnostic accuracy of C - reactive protein and White Cell Count in abdomino-pelvic diseases of non-traumatic acute abdomen by taking CT scan indings as gold standard. We found that CRP levels of above 100 were highly sensitive (94%) and speciic (92%) for an acute surgical abdomen. WCC was not as accurate at determining an acute surgical abdomen due to reasons such as neutropenic sepsis. Research ArticleDiagnostic Accuracy of CRP & WCC in Abdomino-Pelvic Diseases of Non-Traumatic Acute Abdomen by Taking CT Findings as Gold StandardChauhan  MN, Re hm an  S, Riaz M, Jo n ke r L, Ali D, Zam an  S an d Can e lo  R* Department of General & Hepatobiliary Surgery, Cumberland Inirmary Carlisle, UK*Co rre spo n din g autho r: Ruben Canelo FRCS, Department of Hepatobiliary Surgery, Cumberland Inirmary Carlisle, Inirmary Road, Carlisle CA2 7HY, UKRe ce ive d: March 01, 2018; Acce pte d: March 21, 2018; Publishe d: March 28, 2018In tro ductio nAbdominal pain accounts for 5-10% of presentation to the A&E department [1]. he “acute abdomen” is deined as “sudden severe abdominal pain of unknown origin” and is a very common cause for presentation to emergency department worldwide [1-5]. Diagnosis is dependent upon clinical assessment and investigations [3]. Clinical assessment involves a thorough history and clinical examination and is subject to variability based on clinical experience of examining physician [2]. Clinical examination has only been found to be accurate 47-76% of the time with only 50% of junior doctors and doctors working in community reaching a true diagnosis from the above alone [2]. Delay in diagnosis of acute abdomen afects instigation of treatment resulting in increased morbidity and mortality [5-7]. Serological markers of inlammation including C - reactive protein (CRP) and White Cell Count (WCC) are oten used by clinicians as base line investigation to narrow down the diferential diagnosis and guide further investigations including imaging.he role of raise CRP and WCC have been extensively investigated with regards to their role in diagnosing acute appendicitis [3-5]. For example, studies have shown CRP sensitivities in acute appendicitis ranging from 40-94% and speciicities ranging from 38- 87% [3-5]. However, little have been reported regarding the role of these two inlammatory markers in assessing patients presenting with acute abdomen in non-trauma abdomino-pelvic diseases. Sengupta et al. suggest that patients experiencing lower abdominal pain, with normal WCC and CRP, can be sent home [6]. CRP unlike WCC is an ‘add on’ blood marker in many trusts, not used routinely in A&E blood proiles. Acute abdomen is a term frequently used to describe the acute abdominal pain in a subgroup of patients who are seriously ill and have abdominal tenderness and rigidity. Acute abdominal pain or disease may be caused by a myriad of diagnoses, including acute appendicitis, diverticulitis, cholecystitis and perforated bowel [10]. Computed Tomography (CT) of the abdomen and pelvis is considered the most appropriate examination for patient’s abdomino-pelvic diseases [11,12]. Non-enhanced CT, US, and conventional radiography are considered less appropriate initial imaging examinations for these patients [10]. But Ionizing radiation exposure at CT is associated with the risk of radiation induced cancer. his is a drawback of CT, especially as CT is increasingly being used in the diagnostic work-up of young patients [13].Physicians in the emergency department oten base their decisions for consultation of the surgeon for a laparotomy on clinical presentation combined with biochemical abnormalities. Examples of biochemical parameters include raised C-reactive protein (CRP) [14]. C-reactive protein (CRP) is a non-speciic inlammatory marker that is used routinely in many hospitals as an aid in the diagnosis of patients with an acute abdomen [15]. One recent study conclude that biomarkers lactate and CRP in patients with acute abdominal pain should only be used in adjunct to the history and clinical indings, as they are not speciic and can be misleading in establishing a diagnosis. In addition, relying on these biomarkers may contribute to more diagnostic examinations and/or unnecessary invasive interventions (for example laparotomy) [16].Amore recent study reported the sensitivity of CRP (> 5 mg/l) 81.7% and the speciicity 48.5% in patients with abdomino-pelvic diseases, they reported CRP at 48 h ater hospital admission showed a good prognostic accuracy and can be used as an alternative to CT scan [17].he rationale of this study is to determine diagnostic accuracy of C - reactive protein in abdomino-pelvic diseases of non-traumatic acute abdomen taking CT indings as gold standard. here are very limited studies available in the literature with the role of C-reactive in abdomino-pelvic diseases. his study will help us to provide a guide for an early diagnosis, so that patient may have an early diagnosis and successful treatment.his study aimed to evaluate diagnostic accuracy, sensitivity and speciicity of raised CRP and WCC both individually as well Austin J Surg 5(5): id1142 (2018)  - Page - 02Canelo R Austin  Publish in g Gro upSubmit your Manuscript | www.austinpublishinggroup.comas collectively in patients presenting with acute abdomen of non-traumatic origin. Comparison was made with CT scan abdomen indings. Me tho dWe retrospectively evaluated patients admitted with acute abdominal pain to the surgical department between January 2014 to June 2014.Data was entered on a structured proforma. Eligible patients were assessed and an initial diagnosis was made depending on a combination of history, clinical examination, laboratory tests and initial plain radiography. he inal diagnosis was conirmed with CT scan. In total 396 patients were included with all basic demographics, such as name, age and sex being noted. CRP and WCC values within 24 hours of admission were recorded and its diagnostic value was then compared to CT scan results. CRP > 5mg/l and WCC >11 or <4 were set as being abnormal as per standard recommendation and literature reports.he laboratory method used to measure CRP in serum was polyethylene glycol (PEG) enhanced immune-turbid metric assay. he CRP level within 24 hours of admission was recorded and its diagnostic value compared to the inal diagnosis of all patients. Data entry and analysis was performed using SPSS 20. We used chi-square test to compare continuous variable and Mann-Whitney U test for categorical variables.Re su ltsA total of 396 patients fulilled the inclusion criteria; 237 were females and 159 were males with a mean age of 59 years. 74% patient had positive CT scan results i.e. results in actual diagnosis rather than non-speciic abdominal pain.One hundred and ity four cases had abnormal WCC (>11 or <4) and a positive CT scan abdomen indings. he speciicity and sensitivity of WCC was therefore calculated as 72% and 52% respectively. he Pearson Chi-Square: p-value < 0.001 is highly signiicant; i.e. there is a trend that +ve CT is associated with abnormal WCC. Table 1 highlights the speciicity and sensitivity of CRP values only. Both the speciicity and sensitivity increase signiicantly when CRP levels rose above 51. However, CRP levels between 6-50 were only 53% sensitive and 64% speciic. Moreover, Figure 1 demonstrates the increasing positive CT outcome with higher CRP values. At CRP < 5 there are more negative CT scan results shown in blue than positive results, shown in green. A rise in CRP level results in positive CT indings. In addition, Table 2 and Figure 2 above show the speciicity and sensitivity of CRP combined with WCC values. As proved, CRP values were more speciic and sensitive than WCC in the acute abdomen. his is highlighted by when the CT scan result was positive in 26/26 cases where the CRP levels were >200 but with normal WCC. Furthermore, Table 3 below compares sensitivities and speciicities of CRP along with WCC and CRP alone. here is not a signiicant diference in results when CRP levels are between 5-50 with abnormal WCC. But as mentioned previously, as CRP levels raise its signiicance in diagnosing an acute abdomen raises more than a rise in WCC value. Various pathologies were diagnosed during the study following the CT scan report. Table 3 and Figures 3 & 4 below highlight the distribution of pathology. Discuss io nhe acute abdomen remains a diagnostic challenge for many surgeons. Raised CRP and WCC alone cannot indicate whether the cause of abdominal pain may require operative intervention or if it is self-limiting as found recently by Salam et al. [7]. hat is the critism that some studies have issued against using CRP as a diagnostic tool in the surgical abdomen. Andersson et al. in their study reported that Figure 1: Bar chart demonstrating CRP category.Figue 2: Bar chart demonstrating CRP Values.Category (CRP only) Speciicity Sensitivity=<5 n/a n/a6 to 50 64% (72/112) 53% (46/86)51 to 100 85% (53/62) 84% (46/55)101 to 150 92% (36/39) 94% (46/49)151 to 200 94% (34/36) 96% (46/48)200 to 300 98% (56/57) 98% (46/47)>300 97% (33/34) 98% (46/47)Table 1: Values of CRP and Speciicity and Sensitivity.Austin J Surg 5(5): id1142 (2018)  - Page - 03Canelo R Austin  Publish in g Gro upSubmit your Manuscript | www.austinpublishinggroup.comCRP alone was not useful in diferentiating a self-limiting condition like NSAP (non-speciic abdominal pain) from other important surgical causes of acute abdomen [8] whilst surprisingly high levels of CRP was found in patients who would have normally been treated as having NSAP [8]. NSAP is short-lived condition of unknown cause that settles spontaneously with no long-term consequences in majority of the patients at long-term follow-up. High levels of CRP in patients with NSAP may indicate that NSAP is actually an inlammatory condition. However, this adds little or nothing to the fact that such high levels of CRP cannot diferentiate between this self-limiting condition and more potentially serious surgical diagnosis. In our study we are not using CRP or WCC as a diagnostic tool alone. We are using them as an adjunct to reach inal diagnosis which is conirmed by the CT scan abdomen. When used in this manner, we found that CRP can be very useful with high sensitivities and speciicities as mentioned From the results we concluded 2 main points;1. A CRP of 6-50 with abnormal WCC has a better diagnostic value then CRP alone.CRP 6-50 + abnormal WCC = Speciicity 76%, Sensitivity 69%.CRP 6-50 + normal WCC = speciicity 58%, sensitivity 62%.CRP 6-50 (alone) = speciicity 64%, sensitivity 53%.Abnormal WCC alone= speciicity 72%, sensitivity 52%. Category * Speciicity SensitivityCRP <5 and WCC normal (reference category) n/a [reference] n/a [reference]CRP <5 and WCC abnormal 83% (38/46) 20% (2/10)CRP > 5 to 50 and WCC normal 58% (38/66) 62% (45/73)CRP >5 to 50 and WCC abnormal 76% (38/50) 69% (27/39)CRP > 50 to 200 and WCC normal 84% (38/45) 89% (59/66)CRP > 50 to 200 and WCC abnormal 84% (38/45) 91% (69/76)CRP > 200 and WCC normal 100% (38/38) 100% (26/26)CRP > 200 and WCC abnormal 95% (38/40) 97% (56/58)Table 2: CRP levels and speciicity and sensitivity.Diagnosis FrequencyDiverticulitis 36Small bowel obstruction 36Acute Cholecystitis 28Colitis 24Acute pancreatitis 21Dilated CBD +/-  Cholangitis 16Acute Appendicitis 20Tubo ovarian /Adnexal mass 12Colonic malignancy 11Small bowel inlammation (Cohn’s ileitis) 10Intra abdominal  collection 10Negqtive  CT 102Table 3: Frequency of Diagnosis on CT indings.2. CRP alone of 100 or more has better speciicity and sensitivity than other two combinationsCRP 51-100 speciicity = 85%, sensitivity= 84%CRP 101-150 speciicity = 92%, sensitivity =94%CRP>200 = speciicity, sensitivity of 98% each.his shows that CRP levels above 50 have an increased sensitivity and speciicity. he possible reason for CRP being superior to WCC in diagnosing a surgical abdomen is that patients can become neutropenic causing WCC levels to decrease, but CRP remains elevated. Apart from WCC and CRP, lactate levels can also indicate a surgical abdomen. An increase of lactate levels indicates an anaerobicglycogenesis’ and therefore it is a parameter for inadequate perfusion, oxygenation and an estimate of tissue oxygen deiciency. Increased plasma lactate concentrations were observed in patients with mesenteric ischemia with a sensitivity of 100% and a speciicity  Category * Specicity Sensitivity Category  CRP Only Specicity Sensitivity CRP <5 and WCC normal (reference category) n/a [reference]  n/a [reference]     CRP <5 and WCC abnormal 83% (38/46) 20% (2/10)    CRP > 5 to 50 and WCC normal 58% (38/66) 62% (45/73) 6 to 50; 64% (72/112) 53% (46/86) CRP >5 to 50 and WCC abnormal  76% (38/50) 69% (27/39) 6 to 50; 64% (72/112) 53% (46/86) CRP > 50 to 200 and WCC normal 84% (38/45) 89% (59/66)    CRP > 50 to 200 and WCC abnormal 84% (38/45) 91% (69/76)    CRP > 200 and WCC normal 100% (38/38) 100% (26/26) 200 to 300; 98% (56/57) 98% (46/47) CRP > 200 and WCC abnormal 95% (38/40) 97% (56/58) 200 to 300; 98% (56/57) 98% (46/47)  Figure 3: CRP sensitivity and speciicity.Figure 4: CRP values and CT Findings.Austin J Surg 5(5): id1142 (2018)  - Page - 04Canelo R Austin  Publish in g Gro upSubmit your Manuscript | www.austinpublishinggroup.comof 42% [9]. Other studies have shown raised lactate levels can also be due to other surgical conditions such as bowel obstruction and peritonitis. A further study in the comparison of lactate and CRP in the surgical abdomen may prove of beneit. CRP values above 100 are signiicant and patient with such values with a history of acute abdominal pain should undergo a CT scan to further evaluate the cause, which may be surgical or could well be self-limiting.References 1. Emergency education. 2017.2. Ng CS, Watson CJE, Palmer CR, See TC, Beharry NA, Housdon BA, et al. Evaluation of early abdominopelvic computed tomography in patients with acute abdominal pain of unknown cause: prospective randomized study. BMJ. 2002; 325: 1387. 3. Al-Saigh AH. C-reactive protein in the differential diagnosis of the acute abdomen, especially acute appendicitis. J R CollSurgEdinb. 1992; 37: 238-240.4. Gurleyik E, Gurleyik G, Unalmiser S. Accuracy of serum C-reactive protein measurements in diagnosis of acute appendicitis compared with surgeon’s clinical impression. Dis Colon Rectum. 1995; 38: 1270-1274.5. Yang HR, Wang YC, Chung PK, Chen WK, Jeng LB, Chen RJ. Role of leukocyte count, neutrophil percentage, and C-reactive protein in the diagnosis of acute appendicitis in the elderly. Am Surg. 2005; 71: 344-347.6. Sengupta A, Bax G, Paterson-Brown S. White cell count and C-reactive protein measurement in patients with possible appendicitis. Ann R Coll Surg Engl. 2009; 91: 113-115.7. Salem TA, Molloy RG, O’Dwyer PJ. Prospective study on the role of C-reactive protein (CRP) in patients with an acute abdomen. Ann R Coll Surg Engl. 2007; 89: 233-237.8. Andersson RE. Meta-analysis of the clinical and laboratory diagnosis of appendicitis. Br J Surg. 2004; 91: 28-37.9. Lange H, Toivola A. Warning signals in acute abdominal disorders. Lactate is the best marker of mesenteric ischemia. Lakartidningen. 1997; 94: 1893-1896.10. Stoker J, van Randen A, Laméris W, Boermeester MA. Imaging Patients with Acute Abdominal Pain1. Radiology. 2009; 253: 31-46.11. Van Randen A, Laméris W, Nio CY, Spijkerboer AM, Meier MA, Nolthenius CT, et al. Inter-observer agreement for abdominal CT in unselected patients with acute abdominal pain. Eur Radiol. 2009; 19: 1394-1407.12. Matz S, Connell M, Sinha M, Goettl CS, Patel PC, Drachman D. Clinical Outcomes of Pediatric Patients With Acute Abdominal Pain and Incidental Findings of Free Intraperitoneal Fluid on Diagnostic Imaging. J Ultrasound Med. 2013; 32: 1547-1553.13. Stoker J. Magnetic resonance imaging and the acute abdomen. Br J Surg. 2008; 95: 1193-1194.14. Ravishankaran P, Shah AM, Bhat R. Correlation of interleukin-6, serum lactate, and C-reactive protein to inlammation, complication, and outcome during the surgical course of patients with acute abdomen. J  Interferon Cytokine Res. 2011; 31: 685-690.15. Salem T, Molloy R, O’dwyer P. Prospective study on the role of C-reactive protein (CRP) in patients with an acute abdomen. Ann R Coll Surg Engl. 2007; 89: 233-237.16. Meyer ZC, Schreinemakers JM, van der Laan L. The value of C-reactive protein and lactate in the acute abdomen in the emergency department. World Journal of Emergency Surgery. 2012; 7: 22.17. Coyle JP, Brennan CR, Parfrey SF, O’Connor OJ, Mc Laughlin PD, Mc Williams SR, et al. Is serum C-reactive protein a reliable predictor of abdomino-pelvic CT indings in the clinical setting of the non-traumatic acute abdomen?. Emerg radiol. 2012; 19: 455-462.Citation: Chauhan MN, Rehman S, Riaz M, Jonker L, Ali D, Zaman S, et al. Diagnostic Accuracy of CRP & WCC in Abdomino-Pelvic Diseases of Non-Traumatic Acute Abdomen by Taking CT Findings as Gold Standard. Austin J Surg. 2018; 5(5): 1142.Austin J Surg - Volume 5 Issue 5 - 2018ISSN : 2381-9030 | www.austinpublishinggroup.com Canelo et al. © All rights are reserved

Diagnostic accuracy of CRP & WCC in abdomino-pelvic 

diseases of non-traumatic acute abdomen by taking CT 

findings as gold standard

http://insight.cumbria.ac.uk/id/eprint/3848/1/Jonker_DiagnosticAccuracy.pdf

Diagnostic accuracy of CRP & WCC in abdomino-pelvic diseases of non-traumatic acute abdomen by taking CT findings as gold standard

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