Davies, JC

Little is known about the early interactions that occur between respiratory syncytial virus (RSV) infected airway epithelium and NK cells. This includes airway epithelial cell (AEC) surface expression of IL-15/IL-15Ra complex, which could be a potent inducer of NK cell activation via trans-presentation. The work here describes the hypothesis that AECs after infection can activate NK cells which in turn influence immune and inflammatory responses including AEC activity. Human bronchial epithelium cell line, BEAS-2B, and human nasal airway epithelial cells (HNAECs) were used in vitro to characterise the expression of IL-12, IL-15, IL-18 and IL-15Ra during RSV infection. IL-12 protein was not expressed. RSV infection significantly increased expression of cell surface IL-15Ra on BEAS-2B cells and HNAECs. BEAS-2B cells also expressed cell surface IL-15 indicating complex expression. IL-15 protein was only detected in infected BEAS-2B cell culture supernatants and not HNAECs. Soluble IL-18 protein was only expressed by HNAECs and significantly increased with infection. The RSV infected airway epithelium has the potential to activate NK cells with IL-15 signalling predominantly occurring through the IL-15/IL-15Ra ... (continues

Davies, Jennifer Claire

University of Liverpool Repository

The role of airway epithelial cell and natural killer cell interactions during RSV infection

http://livrepository.liverpool.ac.uk/3033634/1/200873240_Mar2019.pdf

The role of airway epithelial cell and natural killer cell interactions during RSV infection

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