Correction for ‘Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine’ by Judith M. Curran et al., Lab Chip, 2010, 10, 1662–1670.</p

Amro, NA

Curran, Judith M

Graham, Duncan

Hunt, John A

Irvine, Eleanore

Jamil, H

Sanedrin, RG

Stokes, Robert

Lab on a Chip

Correction for 'Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine' by Judith M. Curran et al., Lab Chip, 2010, 10, 1662-1670

University of Liverpool Repository

Lab on a ChipCORRECTIONCite this: Lab Chip, 2017, 17, 2135DOI: 10.1039/c7lc90059grsc.li/locCorrection: Introducing dip pen nanolithographyas a tool for controlling stem cell behaviour:unlocking the potential of the next generation ofsmart materials in regenerative medicineJudith M. Curran,*a Robert Stokes,b Eleanore Irvine,b Duncan Graham,b N. A. Amro,cR. G. Sanedrin,c H. Jamilc and John A. HuntaCorrection for ‘Introducing dip pen nanolithography as a tool for controlling stem cell behaviour:unlocking the potential of the next generation of smart materials in regenerative medicine’ by Judith M.Curran et al., Lab Chip, 2010, 10, 1662–1670.The authors regret that errors were made in the construction of Fig. 2 and 3 of this Lab on a Chip article. Specifically theannotation associated with the 140 nm and 1 micron arrays was incorrect. The authors would like to clarify that this minorerror does not change any of the conclusion or discussion points. The authors further regret that subfigures adapted from ref. 1were not attributed by citation to ref. 1 and did not contain credit lines in their captions. The figures and captions have nowbeen updated to correct these errors and are given below.Lab Chip, 2017, 17, 2135–2138 | 2135This journal is © The Royal Society of Chemistry 2017a UKCTE, The Division of Clinical Engineering, School of Clinical Sciences, LINSET, The University of Liverpool, Ground Floor Duncan Building, Daulby Street, Liverpool,UK. E-mail: j.curran@liv.ac.uk; Fax: +44 (0)151 7064915; Tel: Phone: +44 (0)151 7064913bCentre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK.Fax: +44 (0)141 552 0876; Tel: Phone: +44 (0)141 548 4701c NanoInk Inc., 8025 Lamon Ave, Skokie, IL, 60077, USAOpen Access Article. Published on 01 June 2017. Downloaded on 12/07/2017 08:21:15.  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.View Article OnlineView Journal  | View Issue2136 | Lab Chip, 2017, 17, 2135–2138 This journal is © The Royal Society of Chemistry 2017Fig. 2 (A–F) MSC cultured in contact with HDT and MHA modified surfaces for 24 hours and stained with Oregon Green Phallodin (green-stressfibres), vinculin (red-focal contacts) and DAPI (blue-nuclei). Only 280 nm pitch modified surfaces supported viable cell adhesion. (G) Highmagnification image of cells clustered on ODT 280 nm pitch, arrow heads show motile focal adhesions associated with the periphery of thepatterned area, whilst focal adhesions inside the patterned area are dense and well established, depicting the strong binding of the cell via focaladhesions to the underlying nanoarrays. (H) Single frame from time lapse microscopy experiment demonstrating the chemotactic nature of themodified surface. Over a 12 hour time period highlighted cells moved from the periphery of the modified area (where bodies were in contact withcontrol and modified areas) into the centre of the modified area (CH3 280 nm pitch, inside the smaller white square) from the adjacentunmodified areas, full time lapse analysis is available in ESI video S1. The images collected were from low cell density experiments to allowinvestigations into single cell interactions with the surface, the same chemotactic properties were observed when the cell density was increased.Fig. 2A and B are copyright Springer and adapted with permission from ref. 1.Lab on a ChipCorrectionOpen Access Article. Published on 01 June 2017. Downloaded on 12/07/2017 08:21:15.  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.View Article OnlineLab Chip, 2017, 17, 2135–2138 | 2137This journal is © The Royal Society of Chemistry 2017Fig. 3 MSC cultured in contact with MUO (–OH) (A–C) and with AUT (–NH2) (D–H) modified surfaces for 24 hours and stained for F-actin (green),vinculin (red) and cell nuclei (blue). When cultured in contact with 1 μm spaced MUO groups, there was evidence of rounded cell morphology andconcentrated clusters of focal adhesions. Cells cultured in contact with 280 nm and 140 nm pitched surfaces showed parallel fibres of F-actin andan elongated cell morphology with minimal focal contact formation. There was also evidence of concentrated buds of actin on these surfaces (ex-amples of this phenomenon are highlighted by arrow heads). When MSC were cultured in contact with –NH2 modified (D and F) surfaces for 24hours cells were elongated and aligned when cultured in contact with 280 nm pitch surfaces (E), cells in the upper right and left corners are at theouter extremities of the patterned area, therefore show signs of polarisation but are not as strong as cells in the centre of the pattern where theentirety of the cell body receives the same stimulus. Time lapse microscopy (G and H) confirmed the –NH2 280 nm pitch surfaces ability to induceelongation. (G) Single frame from 280 nm pitch –NH2, all of the field of view is patterned with –NH2, there is clear evidence of cell clustering andelongation of the well attached cell clusters, single frame taken from ESI video S2. When the pitch was increased by 100 nm (H) the effect on celladhesion and morphology was significant, at a pitch of 395 nm the cells could not attach properly or elongate and resulted in rounded poorly at-tached cells, the area of modification is located within the four marker points, double letters contained within a square bracket, located at the cor-ners of the field of view, single frame taken from ESI video S3. Fig. 3(D and E) are copyright Springer and adapted with permission from ref. 1.Lab on a Chip CorrectionOpen Access Article. Published on 01 June 2017. Downloaded on 12/07/2017 08:21:15.  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.View Article Online2138 | Lab Chip, 2017, 17, 2135–2138 This journal is © The Royal Society of Chemistry 2017The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.References1 J. M. Curran, R. Chen, R. Stokes, E. Irvine, D. Graham, E. Gubbins, D. Delaney, N. Amro, R. Sanedrin, H. Jamil and J. A. Hunt,J. Mater. Sci.: Mater. Med., 2010, 21, 1021–1029.Lab on a ChipCorrectionOpen Access Article. Published on 01 June 2017. Downloaded on 12/07/2017 08:21:15.  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.View Article Online

Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine (vol 10, pg 1662, 2010)

Judith M. Curran

Robert Stokes

Eleanore Irvine

Duncan Graham

N. A. Amro

R. G. Sanedrin

H. Jamil

John A. Hunt

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Correction: Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine

Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine (vol 10, pg 1662, 2010)

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