Campbell, BJ

Mahalhal, A

Pritchard, DM

Probert, CS

A Mahalhal

BJ Campbell

DM Pritchard

CS Probert

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PTH-051 Influence of Iron Supplementation on The Natural History of Colitis

University of Liverpool Repository

BSG 2016 - Abstract SubmissionInflammatory Bowel DiseaseBSG16-ABS-1423INFLUENCE OF IRON SUPPLEMENTATION ON THE NATURAL HISTORY OF COLITISA. Mahalhal* 1, B. J. Campbell1, D. M. Pritchard1, C. S. Probert11Department of Gastroenterology, University of Liverpool/Institute of Transitional Medicine, Liverpool, United KingdomDoes this abstract contain original data?: YesWill this abstract be published/presented prior to June 2016?: NoThis abstract is: None of the aboveDoes your Endoscopy abstract include a video?: NoPreferred presentation type: Oral or PosterIntroduction: Iron deficiency anaemia is common in IBD. Iron supplementation may induce or exacerbate colitis in rats (APT 2001;15:1989-99).Dysbiosis of the microbiota is common in IBD and iron contributes to this as it is a growth factorfor pathogenic bacteria.We investigated the effect of dietary iron supplementation and/or reduction on the severity ofchronic colitis in a murine model using clinical, histological and biochemical parameters.Methods: Studies were performed on 6 groups of 8 wild type (WT) C57BL/6 mice.Chronic colitis was induced with 1.25%Dextran Sodium Sulphate (DSS) for 5 days,followed by16 further days on water [for 3 consecutive cycles]. DSS-treatedmice were fed one of three diets:low iron [LI] (100ppm), normal iron [NI] (200ppm) and high iron[HI] (400ppm)supplemented chow. Also, 3 non-DSS-treated groups were studied and fed similarly. Half of the mice in each controlgroup were treated with 1 cycle of acute 2% DSS  for 5 days at day 53, followed by 5 further days on water. All mice weresacrificed at day 63. Daily weights and clinical features were recorded. Histological colitis was scored using the Bauerscore(Gut 2010; 59:1192-99).Faecal calprotectin was measured by ELISA and faecal iron by immunoassay at varioustime points [day (d) 1, 21, 42 & 63]. Statistical analyses used the Kruskal–Wallis test with post-hoc analysis.Results: Oral DSS administration induced colitis in all treated mice. While chronic DSS colitis was not associated withweight loss, there was severe weight loss in acute DSS mice which was greatest in the low iron diet group (p=0.001 LI vs.HI; p=0.01 for LI vs. NI). Histologically, the colitis features were more prominent in acute DSS-treated mice ingesting lowand high iron diets, with median colitis scores 6 & 5.5 respectively.Cyclic administration of DSS in drinking water resultedin a significant rise in faecal calprotectin, from baseline to d63 in LI (p=0.05) and for HI (p=0.01), but this was notsignificant in the NI group. In acute DSS, the rise was greater in LI and HI (p=0.001) and less in NI (p=0.01).Total faecaliron was increased in a dose-dependent manner within 9 weeks in all non-DSS groups. Nevertheless, in chronic DSSgroups, p=0.001 at d1 vs. d63 for all groups [382% change for LI, 331% for NI and 355% for HI].However, in acute DSSp=0.05 for LI, p=0.001 NI & HI (d1 vs. d10).Conclusion: Changes in nutritional luminal iron exacerbate colitis.Oral administration of DSS causes a reproducible acutecolitis, followed by a slow recovery phase with a concomitant chronic inflammation. Chronic colitis was worse in mice fedlow or high iron diets, as shown by elevated calprotectin. Faecal iron rose equally in all 3 groups: with iron increases likely arising from diet and bleeding during colitis. Dysbiosis may be a consequence of this change in luminal iron.Disclosure of Interest: None Declared

INFLUENCE OF IRON SUPPLEMENTATION ON THE NATURAL HISTORY OF COLITIS

INFLUENCE OF IRON SUPPLEMENTATION ON THE NATURAL HISTORY OF COLITIS

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