Aldrichina grahami on an aseptic semi-defined diet has been employed to determine the profiles of body weight, protein content, free amino acids and related compounds during metamorphosis. Analysis carried out by means of a automatic amino acid analyzer revealed the presence of thirty-two ninhydrin positive substances in the free amino acid pool of the insect. Among the compounds tyrosine, proline, glutamic acid, aspartic acid and glycine increase abruptly at particular period. Some discussions were done on the physiological significance of the changes in free amino acid concentration during metamorphosis
