Precise calibration of quartz line scales is very important for assuring traceability of microscopic measurements. Very significant influence in calibration uncertainty budget is represented by uncertainty of line centre detection. Line centre is usually detected through video signal processing using different types of algorithms. This paper is presenting calibration procedure that was developed in the Laboratory for Production Measurement at the Faculty of Mechanical Engineering in Maribor. It is focused in uncertainty analysis and especially in the influence of line scale contamination on determination of line centre position. Different types of line scale contamination like dirt spots, scratches, line edge incorrectness, and line intensity were simulated in order to test the ability of the line centre detection algorithm to eliminate such influences from the measurement results
