Supported by the Imperial Cancer Research Fund and by a grant from ‘The Special Trustees of the Middlesex Hospital, University College Hospital and University College London Medical School’. E Passalidou was supported by a Research Fellowship from the European Respiratory Societ

A Cesario

A G Nicholson

A Kolin

A L Harris

AJ Maniotis

BA Warren

BM Almeida

BV Offersen

C Ratcliffe

D Hanahan

DG Tang

DR Coman

DV Parums

E Hyjek

E Navratil

E Passalidou

F Pezzella

H Rabb

I Leigh

J Folkman

J Holash

J Hu

JC Gutheil

K C Gatter

M Ferguson

M Friedlander

M Tetlow

M Trivella

N Nagano

N Singh

P Goldstraw

P Granone

P Wesseling

PC Brooks

S Kakolyris

S Pazouki

U Pastorino

British Journal of Cancer

PubMed

Passalidou, E

Trivella, M

Singh, N

Ferguson, M

Hu, J

Cesario, A

Granone, P

Nicholson, AG

Goldstraw, P

Ratcliffe, C

Tetlow, M

Leigh, I

Harris, AL

Gatter, KC

Pezzella, F

Queen Mary Research Online

Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas

We have previously described a group of non-small cell lung carcinomas without morphological evidence of neo-angiogenesis. In these tumours neoplastic cells fill up the alveoli and the only vessels present appear to belong to the trapped alveolar septa. In the present study we have characterised the phenotype of the vessels present in these non-angiogenic tumours, in normal lung and in angiogenic non-small cell lung carcinomas. The vessels, identified by the expression of CD31, were scored as mature when expressing the epitope LH39 in the basal membrane and as newly formed when expressingaVb3 on the endothelial cells and/or lacking LH39 expression. In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive andaVb3 variable or negative. Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0–60%) expressed LH39, while aVb3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5–90%). We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis. This type of cancer may be resistant to some anti-angiogenic therapy and different strategies need to be developed

Oxford University Research Archive (ORA)

Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.

Passalidou, Elisabeth

Trivella, Maria-Heleni

Singh, Neeta

Ferguson, Michael

Hu, Jiangting

Cesario, Alfredo

Granone, Pierluigi

Nicholson, Andrew G.

Goldstraw, Peter

Ratcliffe, Cathy

Tetlow, Michelle

Leigh, Irene

Harris, Adrian L.

Gatter, Kevin C.

Pezzella, Francesco

ORA - Oxford University Research Archive

We have previously described a group of non-small cell lung carcinomas without morphological evidence of neo-angiogenesis. In these tumours neoplastic cells fill up the alveoli and the only vessels present appear to belong to the trapped alveolar septa. In the present study we have characterised the phenotype of the vessels present in these non-angiogenic tumours, in normal lung and in angiogenic non-small cell lung carcinomas. The vessels, identified by the expression of CD31, were scored as mature when expressing the epitope LH39 in the basal membrane and as newly formed when expressing alphaVbeta3 on the endothelial cells and/or lacking LH39 expression. In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative. Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%). We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis. This type of cancer may be resistant to some anti-angiogenic therapy and different strategies need to be develope

PubliCatt

Vascular phenotype in angiogenic and non angiogenic lung non small cell carcinomas.

Crossref

We have previously described a group of non-small cell lung carcinomas without morphological evidence of neo-angiogenesis. In these tumours neoplastic cells fill up the alveoli and the only vessels present appear to belong to the trapped alveolar septa. In the present study we have characterised the phenotype of the vessels present in these non-angiogenic tumours, in normal lung and in angiogenic non-small cell lung carcinomas. The vessels, identified by the expression of CD31, were scored as mature when expressing the epitope LH39 in the basal membrane and as newly formed when expressing alphaVbeta3 on the endothelial cells and/or lacking LH39 expression. In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative. Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%). We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis. This type of cancer may be resistant to some anti-angiogenic therapy and different strategies need to be developed

Gatter, Kevin

Oxford University Research Archive

A novel lamina lucida component of epithelial and endothelial basement membranes detected by LH39 monoclonal antibody.

Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis.

Relationship of vascular maturation in breast cancer blood vessels to vascular density and metastasis, assessed by expression of a novel basement membrane component, LH39. Br J Cancer 82: 844–851 Kolin A, Koutoulakis T

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2375177

Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas

Abstract

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