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Apoptotic activity is increased in parallel with the metaplasia–dysplasia–carcinoma sequence of the bronchial epithelium
Authors
A Bakhshi
A-K Eerola
+45 more
AJ Minn
C Walker
C Walker
DM Hockenbery
ED King
G Kroemer
G Sozzi
GB Baretton
HB Hellquist
J Yang
JC Reed
JFR Kerr
K Nuorva
K Nuorva
KH Vähäkangas
M Aihara
M Ishida
M Muzio
M Saegusa
MA Birchall
MJ Staunton
MS Greenblatt
N Bardeesy
O Auerbach
O Auerbach
O Auerbach
P Lipponen
P Pääkkö
PK Lipponen
Q-L Lu
S Nagata
SE Bodrug
SW Lowe
T Arai
T Hirano
U Törmänen
U Törmänen
V Sundaresan
WP Bennett
Y Koshida
Y Soini
Y Soini
Y Soini
Y Tsujimoto
Z Oltvai
Publication date
Publisher
Nature Publishing Group
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PubMed
Abstract
A high level of apoptotic activity and an independence of apoptosis from the expression of p53 and bcl-2 have been observed in non-small-cell lung carcinoma. We examined 44 samples of normal, metaplastic and premalignant (i.e. mild, moderate and severe dysplasias and carcinoma in situ) bronchial epithelia to evaluate whether differences in the apoptotic activity could already be seen in the stages preceding squamous cell carcinoma of the lung (SQCLC). Apoptotic cells and bodies were visualized by 3′ end labelling. The expression of p53 and members of the bcl-2 gene family, such as bcl-2, bax and mcl-1, were determined immunohistochemically with specific antibodies. The relative number of apoptotic cells and bodies [apoptotic index (AI%)] was already increased threefold as the normal bronchial epithelium changed to squamous metaplasia, and the AIs of the dysplastic lesions were about four times higher than those of the normal epithelium. Apoptosis was significantly associated with cell proliferation, as determined by proliferating cell nuclear antigen (PCNA) immunohistochemistry. However, the extent of apoptosis did not correlate with the expression of p53, bcl-2, bax and mcl-1. We conclude that, in the metaplasia–dysplasia–carcinoma sequence in the lung, the elevation of the AI% is an early event associated with cell proliferation activity, but is independent of the expression of p53, bcl-2, mcl-1 and bax. © 1999 Cancer Research Campaig
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Last time updated on 04/12/2019