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In vivo fluorescence imaging of the transport of charged chlorine6 conjugates in a rat orthotopic prostate tumour
Authors
A Orenstein
AL Baldwin
+59Β more
AM Dvorak
BA Goff
BW Henderson
BW Henderson
CJ Edgell
D Fukumura
DA Bellnier
DR Senger
E Larson
EF Bernstein
F Yuan
F Yuan
F Zunino
G Bottiroli
G Mariani
G Ojteg
HF Dvorak
HF Dvorak
HF Dvorak
HL van Leengoed
J Kopecek
J Leppala
J Vieweg
JK Frisoli
K Ley
KA Haldenby
LJ Nugent
LR Duska
LW Seymour
LW Seymour
M Masquelier
M Rajadhyaksha
M Simionescu
MR Hamblin
MR Hamblin
N Davis
N Ghinea
N Simionescu
NL Krinick
NS Soukos
PG Calzavara-Pinton
R Bachor
R Duncan
RK Jain
RK Jain
RK Jain
RW Boyle
S Kohn
SK Hobbs
SL Rakestraw
T Hasan
T Hasan
TK Yeung
TP Butler
U Schmidt-Erfurth
V Cuomo
WG Mayhan
WG Roberts
Y Matsumura
Publication date
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Nature Publishing Group
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PubMed
Abstract
Polymeric drug conjugates are used in cancer therapy and, varying their molecular size and charge, will affect their in vivo transport and extravasation in tumours. Partitioning between tumour vasculature and tumour tissue will be of particular significance in the case of photosensitizer conjugates used in photodynamic therapy, where this partitioning can lead to different therapeutic effects. Poly-l-lysine chlorine6 conjugates (derived from polymers of averageMr 5000 and 25 000) were prepared both in a cationic state and by poly-succinylation in an anionic state. A fluorescence scanning laser microscope was used to follow the pharmacokinetics of these conjugates in vivo in an orthotopic rat prostate cancer model obtained with MatLyLu cells. Fluorescence was excited with the 454β528 nm group of lines of an argon laser and a 570 nm long pass filter used to isolate the emission. Results showed that the conjugates initially bound to the walls of the vasculature, before extravasating into the tissue, and eventually increasing in fluorescence. The anionic conjugates produced tissue fluorescence faster than the cationic ones, and surprisingly, the largerMr conjugates produced tissue fluorescence faster than the smaller ones with the same charge. These results are consistent with differences in aggregation state between conjugates. Β© 1999 Cancer Research Campaig
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Last time updated on 04/12/2019