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Expression of SCF splice variants in human melanocytes and melanoma cell lines: potential prognostic implications
Authors
Artuc M
Bosenberg MW
+46 more
Cohen PS
Costa JJ
D Schadendorf
Dippel E
Flanagan JG
Grabbe J
Grabbe J
Grichnik JM
Guo CS
Hamann K
Hamann K
Hartmann K
Herlyn M
Herlyn M
Huang EJ
Huang S
Inoue M
J Grabbe
Lahav R
Longley BJ
Longley BJ
Lu C
Luo D
M Artuc
Majumdar MK
Miura N
Natali PG
P Welker
Papadimitriou CA
Ramenghi U
Ratajczak MZ
Schadendorf D
Schadendorf D
Schadendorf D
Schadendorf D
Scott G
Sviderskaya EV
Takahashi H
Tokunaga K
Turner AM
V Langrish
Van Muijen GN
Wehrle Haller B
Welker P
Worm M
Zsebo KM
Publication date
Publisher
Nature Publishing Group
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on
PubMed
Abstract
Stem cell factor (SCF), the ligand for c-Kit, is known to regulate developmental and functional processes of haematopoietic stem cells, mast cells and melanocytes. Two different splice variants form predominantly soluble (sSCF or SCF-1) and in addition some membrane-bound SCF (mSCF or SCF-2). In order to explore the prognostic significance of these molecules in melanoma, total SCF, SCF splice variants and c-Kit expression were studied in normal skin melanocytes and in 11 different melanoma cell lines, using reverse transcription polymerase chain reaction, immunocytochemistry and enzyme-linked immunosorbent assay. Nine of the 11 melanoma cell lines expressed SCF-1 mRNA, only two of them SCF-2, and these two also SCF-1. Coexpression of both SCF-1 and c-Kit was noted in five cell lines, and only one cell line as well as normal melanocytes expressed both SCF-1 and SCF-2 as well as c-Kit. Corresponding results were obtained on immunocytochemical staining. Of three exemplary melanoma cell lines studied, two expressing SCF mRNA also released SCF spontaneously and on stimulation, whereas the line lacking SCF and c-kit mRNA (SK-Mel-23) failed to do so. These data demonstrate thus that melanoma cell lines, particularly those known to metastasize in vivo, lose the ability to express SCF-2 mRNA, suggesting that this molecule may serve, next to c-Kit, as a prognostic marker for malignant melanoma. © 2000 Cancer Research Campaig
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info:doi/10.1054%2Fbjoc.1999.1...
Last time updated on 11/12/2019