 gene.-exposed women than from men.These findings suggest that other parasite proteins, such as PFI1785w, may contribute beside VAR2CSA to the pathogenesis of PAM. These data may be very valuable for future vaccine development

A Craig

A Salanti

Ali Salanti

Alioune Gaye

AS Gowda

AT Jensen

B Maubert

BJ Brabin

Caroline Proux

CH Ricke

CJ Treutiger

CV Plowe

E Lasonder

Emmanuel Bischoff

HM Kyriacou

James Beeson

Jean-Yves Coppée

JG Beeson

JM Moore

JP Daily

Juliette Guitard

L Florens

M Fried

MF Duffy

Morten A. Nielsen

NG Tuikue Ndam

Nicaise Tuikue Ndam

NK Viebig

Peter H. David

Philippe Deloron

PR Sanders

RC Gentleman

RN Haase

S Jafari-Guemouri

S Kyes

SA Ralph

SE Francis

T Staalsoe

Thomas Lavstsen

Thor Theander

TJ Sargeant

W Trager

PLoS ONE

English

PubMed

Tuikue Ndam, Nicaise

Bischoff, E.

Proux, C.

Lavstsen, T.

Salanti, A.

Guitard, Juliette

Nielsen, M.A.

Coppée, J.Y.

Gaye, A.

Theander, T.

David, P.H.

Deloron, Philippe

Name not available

Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression

Crossref

Plasmodium falciparum Transcriptome Analysis Reveals Pregnancy Malaria Associated Gene Expression

BackgroundPregnancy-associated malaria (PAM) causing maternal anemia and low birth weight is among the multiple manifestations of Plasmodium falciparum malaria. Infected erythrocytes (iEs) can acquire various adhesive properties that mediate the clinical severity of malaria. Recent advances on the molecular basis of virulence and immune evasion have helped identify var2csa as a PAM-specific var gene.Methodology/principal findingsThe present study presents a genome-wide microarray transcript analysis of 18 P. falciparum parasite isolates freshly collected from the placenta. The proportion of PAM over-expressed genes located in subtelomeric regions as well as that of PAM over-expressed genes predicted to be exported were higher than expected compared to the whole genome. The identification of novel parasite molecules with specificity to PAM and which are likely involved in host-pathogen interactions and placental tropism is described. One of these proteins, PFI1785w, was further characterized as the product of a two-exon PHIST gene, and was more often recognized by serum samples from P. falciparum-exposed women than from men.Conclusions/significanceThese findings suggest that other parasite proteins, such as PFI1785w, may contribute beside VAR2CSA to the pathogenesis of PAM. These data may be very valuable for future vaccine development

Morten A Nielsen

Peter H David

Directory of Open Access Journals

Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression.

Tuikue Ndam Nicaise

Bischoff Emmanuel

Proux Caroline

Lavstsen Thomas

Salanti Ali

Guitard Juliette

Nielsen Morten A.

Coppée Jean-Yves

Gaye Alioune

Theander Thor

David Peter H.

Deloron Philippe

Public Library of Science (PLOS)

 Transcriptome Analysis Reveals Pregnancy Malaria Associated Gene Expression

Bischoff, Emmanuel

Proux, Caroline

Lavstsen, Thomas

Salanti, Ali

Nielsen, Morten A

Coppée, Jean-Yves

Gaye, Alioune

Theander, Thor

David, Peter H

Copenhagen University Research Information System

<em>Plasmodium falciparum</em> transcriptome analysis reveals pregnancy malaria associated gene expression

A proteomic view of the Plasmodium falciparum life cycle.

A simple perfusion technique for isolation of maternal intervillous blood mononuclear cells from human placentae.

A single member of the Plasmodium falciparum var multigene family determines cytoadhesion to the placental receptor chondroitin sulphate A.

Adherence of Plasmodium falciparum to chondroitin sulfate A in the human placenta.

Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry.

Antibodies among men and children to placental-binding Plasmodium falciparum-infected erythrocytes that express var2csa.

Antigenic variation at the infected red cell surface in malaria.

Bioconductor: open software development for computational biology and bioinformatics.

Broad analysis reveals a consistent pattern of var gene transcription in Plasmodium falciparum repeatedly selected for a defined adhesion phenotype.

Detection of antobodies to variant surface antigens on Plasmodium falciparum-infected erythrocytes by flow cytometry.

Development of antibodies against chondroitin sulfate A-adherent Plasmodium falciparum in pregnant women.

Differential var gene transcription in Plasmodium falciparum isolates from patients with cerebral malaria compared to hyperparasitaemia.

Distinct proteins classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum.

Dynamics of anti-VAR2CSA immunoglobulin G response in a cohort of senegalese pregnant women.

Evidence for the involvement of VAR2CSA in pregnancy-associated malaria.

High Level of var2csa Transcription by Plasmodium falciparum Isolated from the Placenta.

Human malaria parasites in continuous culture.

In vitro selection of Plasmodium falciparum 3D7 for expression of variant surface antigens associated with severe malaria in African children.

In vivo transcriptome of Plasmodium falciparum reveals overexpression of transcripts that encode surface proteins.

Lineagespecific expansion of proteins exported to erythrocytes in malaria parasites.

Maternal antibodies block malaria.

Maternal malaria and parasite adhesion.

PECAM-1/CD31, an endothelial receptor for binding Plasmodium falciparuminfected erythrocytes.

Plasma antibodies from malaria-exposed pregnant women recognize variant surface antigens on Plasmodium falciparum-infected erythrocytes in a parity-dependent manner and block parasite adhesion to chondroitin sulfate A.

Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by group A var genes.

Plasmodium falciparum genotype population dynamics in asymptomatic children from Senegal.

Plasmodium falciparum isolates from infected pregnant women and children are associated with distinct adhesive and antigenic properties.

Plasmodium falciparum parasites expressing pregnancy-specific variant surface antigens adhere strongly to the choriocarcinoma cell line BeWo.

Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa.

Selective upregulation of a single distinctly structured var gene in chondroitin sulphate Aadhering Plasmodium falciparum involved in pregnancy-associated malaria.

Six genes are preferentially transcribed by the circulating and sequestered forms of Plasmodium falciparum parasites that infect pregnant women. Infect Immun.

Structural basis for the adherence of Plasmodium falciparum-infected erythrocytes to chondroitin 4-sulfate and design of novel photoactivable reagents for the identification of parasite adhesive proteins.

The distinct proteome of placental malaria parasites.

The sick placenta-the role of malaria.

Transcribed var genes associated with placental malaria in Malawian women.

Transcriptome analysis of antigenic variation in Plasmodium falciparum–var silencing is not dependent on antisense RNA.

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Plasmodium falciparum Transcriptome Analysis Reveals Pregnancy Malaria Associated Gene Expression

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Crossref

Directory of Open Access Journals

Public Library of Science (PLOS)

Copenhagen University Research Information System

Public Library of Science (PLOS)