Abstract Background The homeodomain transcription factor IPF1/PDX1 exerts a dual role in the pancreas; <it>Ipf1/Pdx1 </it>global null mutants fail to develop a pancreas whereas conditional inactivation of <it>Ipf1/Pdx1 </it>in β-cells leads to impaired β-cell function and diabetes. Although several putative target genes have been linked to the β-cell function of <it>Ipf1/Pdx1</it>, relatively little is known with respect to genes regulated by IPF1/PDX1 in early pancreatic progenitor cells. Results Microarray analyses identified a total of 111 genes that were differentially expressed in e10.5 pancreatic buds of <it>Ipf1/Pdx1</it>-/- embryos. The expression of one of these, <it>Spondin 1</it>, which encodes an extracellular matrix protein, has not previously been described in the pancreas. Quantitative real-time RT-PCR analyses and immunohistochemical analyses also revealed that the expression of <it>FgfR2IIIb</it>, that encodes the receptor for FGF10, was down-regulated in <it>Ipf1/Pdx1</it>-/- pancreatic progenitor cells. Conclusion This microarray analysis has identified a number of candidate genes that are differentially expressed in <it>Ipf1/Pdx1</it>-/- pancreatic buds. Several of the differentially expressed genes were known to be important for pancreatic progenitor cell proliferation and differentiation whereas others have not previously been associated with pancreatic development.</p

Bergqvist, Ingela

Edlund, Helena

Lundeberg, Joakim

Rydén, Patrik

Svensson, Per

Williams, Cecilia

English

PubMed

Svensson, P

Williams, C,

Lundeberg, J

Ryden, P

Bergqvist, I

Edlund, H

Swepub

Gene array identification of Ipf1/Pdx1-/- regulated genes in pancreatic progenitor cells

Per Svensson

Cecilia Williams

Joakim Lundeberg

Patrik Rydén

Ingela Bergqvist

Helena Edlund

Springer - Publisher Connector

Gene array identification of Ipf1/Pdx1-/- regulated genes in pancreatic progenitor cells

Background: The homeodomain transcription factor IPF1/PDX1 exerts a dual role in the pancreas; Ipf1/Pdx1 global null mutants fail to develop a pancreas whereas conditional inactivation of Ipf1/Pdx1 in beta-cells leads to impaired beta-cell function and diabetes. Although several putative target genes have been linked to the beta-cell function of Ipf1/Pdx1, relatively little is known with respect to genes regulated by IPF1/PDX1 in early pancreatic progenitor cells. Results: Microarray analyses identified a total of III genes that were differentially expressed in e10.5 pancreatic buds of Ipf1/Pdx1(-/-) embryos. The expression of one of these, Spondin 1, which encodes an extracellular matrix protein, has not previously been described in the pancreas. Quantitative real-time RT-PCR analyses and immunohistochemical analyses also revealed that the expression of FgfR2IIIb, that encodes the receptor for FGF10, was down-regulated in Ipf1/Pdx1(-/-) pancreatic progenitor cells. Conclusion: This microarray analysis has identified a number of candidate genes that are differentially expressed in Ipf1/Pdx1(-/-) pancreatic buds. Several of the differentially expressed genes were known to be important for pancreatic progenitor cell proliferation and differentiation whereas others have not previously been associated with pancreatic development.</p

Williams, Cecilia,

Lundeberg, Joakim,

Ryden, Patrik

Gene array identification of Ipf1/Pdx1(-/-) regulated genes in pancreatic progenitor cells

Svensson, Per,

Ryden, Patrik,

Edlund, Helena,

Gene array identification of Ipf1/Pdx1 -/- regulated genes in pancreatic progenitor cells

Crossref

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Gene array identification of Ipf1/Pdx1-/- regulated genes in pancreatic progenitor cells

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