The denaturation of transferrin by urea has been studied by (a) electrophoresis in
polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in
iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein
were found to denature at different urea concentrations
