A Inoue

A Joutel

AC Oates

AM Bailey

Ashok Hegde

AW Lowe

B Appel

B Sosa-Pineda

B Thisse

BB Riley

BJ Thompson

C Brou

C Takke

C Thisse

C Zhang

D Jülich

D Leake

DJ Hill

DK Irvin

E Zecchin

EC Lai

F Esni

Felicia Soo Lee Ng

FJM van Eeden

FM Lu

G Gradwohl

G Gu

G Mellitzer

G Struhl

GB Willer

Geraldine Butler

H Hirata

H Huang

H Wan

H Yang

HC Park

HJ Kim

J Jensen

J Kohyama

J Xu

JF Habener

JM Pleau

Joshy George

JS Mumm

Kenny Qi-Ye Tay

Kunde Ramamoorthy Govindarajan

KW Yoo

L Chang

L Li

L Qiu

L St-Onge

LE Matesic

LW Ellisen

M Amoyel

M Itoh

M Lecourtois

M Ma

M Olbrot

M Schena

M Schäfer

M Takamiya

M Tsutsumi

MB Eisen

ME Fortini

MI Mally

MK Chiang

MP Bulman

Nick Chuanxin Qiu

NS Yee

O Pourquié

PCG Rida

R Kopan

R McDaniell

S Artavanis-Tsakonas

S Hans

S Jarriault

S Kidd

S Mathavan

S-W Chong

SA Holley

SA Hutchinson

SH Lam

Sinnakaruppan Mathavan

SL Pfaff

SS Shankaran

Steven Hao-Kee Ho

T Oda

T Ohtsuka

TW van Haeften

VG Tusher

X Qiu

English

PubMed

 gene, which encodes a RING E3 ligase required for Notch activation via Delta/Jagged ubiquitylation and internalization. hybridization were largely consistent with microarray data. mutants generating useful genomic resources and providing an opportunity to identify the function of novel genes involved in Notch signaling and Notch-regulated developmental processes

Hegde Ashok

Qiu Nick Chuanxin

Qiu Xuehui

Ho Steven Hao-Kee

Tay Kenny Qi-Ye

George Joshy

Ng Felicia Soo Lee

Govindarajan Kunde Ramamoorthy

Gong Zhiyuan

Mathavan Sinnakaruppan

Jiang Yun-Jin

Public Library of Science (PLOS)

 Alleles with Pancreas Defects of Different Severity Identifies Putative Notch Responsive Genes

BACKGROUND:Notch signaling is an evolutionarily conserved developmental pathway. Zebrafish mind bomb (mib) mutants carry mutations on mib gene, which encodes a RING E3 ligase required for Notch activation via Delta/Jagged ubiquitylation and internalization. METHODOLOGY/PRINCIPAL FINDINGS:We examined the mib mutants for defects in pancreas development using in situ hybridization and GFP expression analysis of pancreas-specific GFP lines, carried out the global gene expression profile analysis of three different mib mutant alleles and validated the microarray data using real-time PCR and fluorescent double in situ hybridization. Our study showed that the mib mutants have diminished exocrine pancreas and this defect was most severe in mib(ta52b) followed by mib(m132) and then mib(tfi91), which is consistent with the compromised Notch activity found in corresponding mib mutant alleles. Global expression profile analysis of mib mutants showed that there is a significant difference in gene expression profile of wt and three mib mutant alleles. There are 91 differentially expressed genes that are common to all three mib alleles. Through detailed analysis of microarray data, we have identified several previously characterized genes and some putative Notch-responsive genes involved in pancreas development. Moreover, results from real-time PCR and fluorescent double in situ hybridization were largely consistent with microarray data. CONCLUSIONS/SIGNIFICANCE:This study provides, for the first time, a global gene expression profile in mib mutants generating useful genomic resources and providing an opportunity to identify the function of novel genes involved in Notch signaling and Notch-regulated developmental processes

Xuehui Qiu

Zhiyuan Gong

Yun-Jin Jiang

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