Conferencia sobre aproximaciones sistémicas al conocimiento biológicoGene Regulatory Networks controlling Arabidopsis Root Stem Cells
Identifying the transcription factors (TFs) and associated regulatory processes involved in stem
cell regulation is key for understanding the initiation and growth of tissues and organs. Although
many TFs have been described in the Arabidopsis root stem cells, a comprehensive view of the
transcriptional signature of the stem cells is lacking. We used a systems biology approach to
predict interactions among the genes involved in stem cell identity and maintenance.
We first transcriptionally profiled four stem cell populations and developed a gene regulatory
network (GRN) inference algorithm, GENIST, which combines spatial and temporal transcriptomic
datasets to identify important TFs and infer gene-to-gene interactions. Our approach resulted in a
map of gene interactions that orchestrates the transcriptional regulation of stem cells. In addition
to linking known stem cell factors, our resulting GRNs predicted additional TFs involved in stem
cell identity and maintenance. We mathematically modeled and experimentally validated some of
our predicted transcription factors, which confirmed the robustness of our algorithm and our
resulting networks. Our approach resulted in the finding of a factor, PERIANTHIA (PAN), which
may play an important role in stem cell maintenance and QC function.
We then developed an imaging system to perform in vivo, long-term imaging experiments that will
be used to understand the dynamics of the regulatory interactions between PAN and its
downstream TFs in a cell-specific manner. For this, we designed and 3-D printed a Multi-sample
Arabidopsis Growth and Imaging Chamber (MAGIC) that provides near-physiological imaging
conditions and allows high-throughput time-course imaging experiments in the ZEISS Lightsheet
Z.1. We showed MAGIC’s imaging capabilities by following cell divisions, as an indicator of plant
growth and development, over prolonged time periods, and demonstrated that plants imaged with
our chamber undergo cell divisions for >16 times longer than those with the glass capillary
system supplied by the ZEISS Z1. Future in vivo observations of the expression of PAN and its
predicted downstream factors will be key to refine our model predictions and obtain information
about the dynamics of the regulatory processes.
Our systems biology approach illustrates the strength of integrating computational and
technological tools into the experimental approaches to solve key biological questions. We
anticipate that our algorithm and our approach can be applied to solve similar problems in a
diverse number of systems, which can result in unsupervised predictions of gene functions and
gene candidates.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
