Poster presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research, 27-28 November 2015, Egas Moniz, Caparica, Portugal

Carvalho, Joana

Félix, Sérgio

Neto, Bruno

Oom, Madalena

Repositório Comum

!!Conclusion !!The	  type	  of	  prosthesis	  does	  not	  seem	  to	  inﬂuence	  the	  coloniza6on	  by	  yeast!!!! !!!!Oral Fungal Microflora !Associated With Implant-Supported VS.! Mucous-Supported Dentures!!C?RE	  	  ??????????? ????????????????????????????Introduction!	  Nowadays,	  the	  total	  implant-­‐supported	  prosthesis	  is	  assumed	  as	  the	  gold	  standard	   for	   the	   total	   edentulous.	   Yet,	   not	   knowing	   the	   type	   of	   fungal	  ﬂora	   may	   diﬃcult	   the	   applica6on	   of	   a	   therapeu6c	   solu6on	   in	   case	   of	  fungal	   infec6on	   [1].	   Prostheses	   are	   a	  microbiological	   reservoir,	   crea6ng	  an	   addi6onal	   surface	   for	   plaque	   adhesion	   which	   triggers	   a	   nega6ve	  response	  from	  the	  individual’s	  mucosa	  [2].	  Candida	  albicans	  is	  known	  as	  the	  most	  prevalent	  fungus	  in	  the	  oral	  cavity	  [3]	  and	  many	  studies	  report	  infec6ons	  by	   this	  microorganism	   [4].	  Therefore,	   it	   is	   important	   to	   study	  its	  prevalence	   in	   this	  new	  prosthe6c	   rehabilita6on	  and	  compare	   it	  with	  the	  tradi6onal	  total	  acrylic	  prosthesis,	  which	  is	  the	  most	  common	  type	  of	  prosthe6c	  rehabilita6on.	  	  Goals !!Compare	   the	   prevalence	   of	   yeast	   present	   in	   total	   implant-­‐supported	  (TISP)	  and	  total	  mucous-­‐support	  prosthesis	  (TMSP).	  !!!!!!Materials and Methods !!30	  pa6ents	  rehabilitated	  with	  total	  implant-­‐supported	  dentures,	  chosen	  by	   convenience	   were	   observed.	   Samples	   were	   collected	   in	   the	   area	  adjacent	  to	  the	  prostheses	  with	  a	  sterile	  swab	  and	  in	  the	  periimplantar	  sulcus	   of	   greater	   depth	   with	   a	   sterile	   paper	   cone,	   which	   were	  immediately	   analysed	   for	   the	   presence	   of	   yeast.	   The	   yeasts	   were	  isolated	  and	  iden6ﬁed	  in	  BrillianceTM	  Candida	  agar.	   !!!!!Results and Discussion!	  	  A	  TISD	  group	  of	  30	  subjects,	  comprising	  31%	  men	  and	  69%	  women	  with	  an	  average	  age	  of	  64.8	  ±11	  years,	  was	  compared	  with	  a	  group	  of	  60	  subjects	  wearing	  total	  mucous-­‐supported	  dentures	  comprising	  35	  %	  men	  and	  65%	  women	  with	  an	  average	  age	  of	  65.3	  ±11years.	  The	  rela6ve	  coloniza6on	  of	  both	  types	  of	  prostheses	  (mucous	  and	  implant)	  were	  evaluated	  by	  the	  qui-­‐square	  test.	  	  	  No	  signiﬁcant	  diﬀerences	  were	  found	  (p>0.05)	  in	  the	  coloniza6on	  of	  TISD	  (63%	   of	   the	   subjects	   colonized)	   and	   TMSD	   (58%)	   (ﬁgure	   1).	   Equally,	   no	  diﬀerences	   were	   detected	   when	   the	   simultaneous	   coloniza6on	   by	   one,	  two,	   or	  more	   diﬀerent	   yeast	   were	   compared.	   C.	   albicans	   was	   the	  most	  prevalent	   species	   detected	   in	   72%	   of	   the	   TISD	   and	   74%	   of	   the	   TMSD	  (ﬁgure	  2),	  leading	  to	  the	  conclusion	  that	  the	  mode	  of	  prosthe6c	  support	  is	  not	   a	   risk	   factor	   for	   coloniza6on	   with	   this	   species.	   Within	   the	   non-­‐C.	  albicans	   species	  we	   found	   in	   TISD	  C.	   glabrata	   (55%),	  C.	   krusei	   (22%),	  C.	  tropicallis	   (11%)	   and	   other	   3	   species	   (11%)	   while	   in	   the	   TMSD,	   only	   3	  species	   were	   found	   C.	   glabrata	   (45%),	   C.	   tropicallis	   (45%)	   and	   C.	   krusei	  (10%)	   (ﬁgure	  3).	  Apparently,	   the	   implant–support	  dentures	   are	  prone	   to	  	  coloniza6on	  by	  more	  species	  of	  Candida.	  !! !!!Bruno	  Neto1,	  Joana	  Carvalho2,	  Madalena	  Oom3	  and	  Sérgio	  Felix3	  	  1.	  DDS,	  monitor	  of	  Oral	  Rehabilita6on,	  Integrated	  Master's	  Degree	  in	  Den6stry	  ISCSEM,	  Quinta	  da	  Granja,	  Monte	  de	  Caparica	  -­‐	  Portugal	  2.	  DDS,	  assistant	  of	  Oral	  Rehabilita6on,	  Integrated	  Master's	  Degree	  in	  Den6stry	  ISCSEM,	  Quinta	  da	  Granja,	  Monte	  de	  Caparica	  -­‐	  Portugal	  	  3.	  PhD	  Prof.	  Health	  Sciences	  Ins6tute,	  CiiEM	  ISCSEM,	  Quinta	  da	  Granja,	  Monte	  de	  Caparica	  -­‐	  Portugal.	  	  	  References !	  [1]	  Hyland,	  R.,	  Ellis,	  J.,	  Thomason,	  M.,	  El-­‐Feky,	  A.,	  &	  Moynihan,	  P.	  (2009).	  A	  qualita6ve	  study	  on	  pa6ent	  perspec6ves	  of	  how	  conven6onal	  and	  implant-­‐supported	  dentures	  aﬀect	  ea6ng.	  Journal	  of	  Den6stry,	  [2]	  Real-­‐Osuna,	  J.,	  Almendros-­‐Marques,	  N.	  and	  Gay-­‐Escoda,	  C.	  (2012).	  Prevalence	  of	  complica6ons	  aler	  the	  oral	  rehabilita6on	  with	  implant-­‐supported	  hybrid	  prostheses.	  Medicina	  Oral	  Patología	  Oral	  Y	  Cirugia	  Bucal,	  17(1),	  e116–e121	  [3]	  McManus,	  B.	  and	  Coleman,	  D.	  C.	  (2014).	  Molecular	  epidemiology,	  phylogeny	  and	  evolu6on	  of	  Candida	  albicans.	  Infec6on,	  Gene6cs	  and	  Evolu6on!:	  Journal	  of	  Molecular	  Epidemiology	  and	  Evolu6onary	  Gene6cs	  in	  Infec6ous	  Diseases,	  21,166	  [4]	  Manfredi	  et	  al.	  (2013).	  Urban	  legends	  series:	  oral	  candidosis.	  Oral	  Diseases,	  19(3),	  245–61.	  	  Chart.1	  –	  Comparison	  of	  yeast	  coloniza6on	  in	  TISP	  and	  TMSP	  	  Figure	  3	  –	  Comparison	  of	  non-­‐C.	  albicans	  	  between	  TISP	  and	  TMSP	  	  0%#10%#20%#30%#40%#50%#60%#70%#80%#TISP% TMSP%72%% 74%%28%% 26%%Comparison%of%Candida%species%&C.%abicans%C.%non%albicans%0%#10%#20%#30%#40%#50%#60%#70%#80%#90%#100%#TISP% TMSP%37%% 42%%63%% 58%%Comparison%of%yeast%coloniza?on%colonized%not%colonized%Figure	  2	  –	  Comparison	  of	  Candida	  species	  between	  TISP	  and	  TMSP	  	  0%#10%#20%#30%#40%#50%#60%#TISP% TMSP%55%%45%%22%%10%%11%%45%%11%%0%Comparison%of%%non#C.&albicans&&coloniza:on%C.#glabrata#C.#krusei#C.#tropicallis#Others#

Oral fungal microflora associated with implant-supported VS. mucous-supported dentures

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Oral fungal microflora associated with implant-supported VS. mucous-supported dentures

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