Communication presented at the TEMPH 2014 - Trends in Environmental Microbiology for Public Health. Lisbon, 18-21 September 2014

Barroso, Helena

Duarte, Aida

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Microbiological quality assessment of sand from beaches in Portuguese coast: 15 years of experienceHelena BarrosoCiiEM, Instituto Superior de Ciências da Saúde Egas MonizLaboratório de Microbiologia Aplicada Egas MonizAida DuarteiMed, Faculdade de Farmácia da Universidade de LisboaHelena Barroso Temph2014Helena Barroso Temph2014Sources ofcontaminationHelena Barroso Temph2014Quality indicators:Bacteria Indicators of Human ContaminationTotal coliforms, Escherichia coli , Faecal EnterococciPotential Pathogenic BacteriaStaphylococcus spp. coagulase positive, PseudomonasaeruginosaYeastsCandida albicansHelena Barroso Temph2014MoldsAspergillus niger, Aspergillus fumigatus, Penicillium spp.,Alternaria spp., Cladosporium spp., Fusarium spp., Mucor spp.Scytalidium spp, Scopulariopsis spp and DermatophitesHelena Barroso Temph2014The analytical procedures, applied to the sand washingsolution, were adapted from reference methods used inbacteriological analysis of recreational water.Different selective culture media are used for the isolationand characterization of the microorganisms.Helena Barroso Temph2014Microbial control of the beach sand duringbathing seasonIMPORTANTHelena Barroso Temph2014Helena Barroso Temph2014020406080100120140160180200220240SecaHúmida583 >1000Sum of the number of bacteria surveyed in the dry and wetarea in the samples taken during the 2001 bathing season1        2       3       4        5       6       7       8        9Helena Barroso Temph20141        2      3        4        5       6       7       8      9Sum of the number of fungi surveyed in the dry and wetarea in the samples taken during the 2001 bathing season051015202530354045505560SecaHúmida> 60Helena Barroso Temph2014 Harvests made  at 3 points in the dry sand Microbial control should be done before the beginning of the bath season and during all summer. The municipalities responsible for beaches, promote the cleaning and in some cases even the disinfection of sand.Helena Barroso Temph2014Bacteria found in more quantity- Total coliforms- Faecal Enterococci- Staphylococcus spp- Bacillus sppBacteria found in small quantity- Pseudomonas aeruginosa- Staphylococcus coagulase positiveHelena Barroso Temph2014020406080100120Escherichia coli Enterococos fecais PseudomonasaeruginosaStaphylococcusspp coagulase +71030 0UFC/gSum of the number of bacteria surveyed in thesamples taken during the 2013 bathing seasonHelena Barroso Temph2014Given the results of mycological control, the best indicators of pollution from the sands of the beaches are:Filamentous fungi (Molds)Fusarium, Aspergillus niger spp, Mucor spp, Alternaria sppYeasts.Candida albicansHelena Barroso Temph2014Aspergillus fumigatus is almost absentCladosporium spp and Penicillium spp  very commonnormal flora in the environmentHelena Barroso Temph2014First years the number of isolates was higherActually the number of isolates is low, except in the firstsample, before treatmentIt is possible to have a low contamination during all bathseasonPeaks of contamination can occur after days of rain  mostcommon microrganism faecal EnterococciHelena Barroso Temph201405101520253035UFC/gAmostrasNumber of isolates surveyed in samples in eachbeach taken during the 2013 bathing seasonHelena Barroso Temph2014Sanitary management of beaches can play a very important role with regard to public health.Sanitation and disinfection done by specialist is important to maintain a good quality of sand.Helena Barroso Temph2014Thank you for you attentionSr. António Bagulho

Microbiological quality assessment of sand from beaches in Portuguese coast: 15 years of experience

https://comum.rcaap.pt/bitstream/10400.26/9752/1/ProfHBarroso_AprOral01.pdf

Microbiological quality assessment of sand from beaches in Portuguese coast: 15 years of experience

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