Poster presented at the First International Congress of CiiEM  - From Basic Sciences To Clinical Research. Egas Moniz, Caparica, Portugal, 27-28 November 201

Barahona, Isabel

Caldeira, Jorge

Catrau, Mafalda

Félix, Sérgio

Marques, Rita

English

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HEMATEGDMAMMAUDMAHPLC quantitative determination of acrylic monomers released from resins used in Dental RestorationCentro de investigaçãointerdiciplinarEgas Moniz, InstitutoSuperiorde Ciências da SaúdeEgas Moniz , 2829 - 511 Caparica-Portugalibarahona@egasmoniz.edu.ptMafalda Catrau, Rita Marques, Sergio Félix, Jorge Caldeira , and Isabel BarahonaaINTRODUCTIONThe polymerization in dental resins is not completed, thereforemonomers are released to the environment. Due to the fact some ofthese monomers are toxic and have side effects in tissues, it isfundamental to characterized the resin. Hence, HPLC is commonlyused to identify and quantify the molecules which dental material´sare made of.OBJECTIVEOptimize and validate an analytical HPLC method for the identificationand quantification of some monomers present in the temporary dentalprostheses resins TAB 2000®, Protemp 4TM e Structur 3®.METHODSFive solutions at different concentrations were made from thestandard solutions of the monomers hydroxyethyl methacrylate(HEMA), triethylene glycol-dimethacrylate (TEGMA), methylmethacrylate (MMA) and urethane dimethacrylate (UDMA), wich wereanalyzed by RP-HPLC, obtaining the good calibration curve. Sampleswere prepared according to the manufacturers´ instructions by a sink-in method, for periods of 24 hours, 7 days and 14 days.RESULTSThe separation and identification of the monomers was possible asthe constancy in retention times. TAB 2000® resin is made of MMAand TEGMA; Protemp 4TM is made of TEGMA and UDMA; andStructur 3® is made of TEGMA, MMA and UDMA. TAB 2000® is theone with the highest concentration of eluted monomers. UDMAconcentration is higher on Structur 3®. In the first 24 hours, theconcentration variation of all Structur 3® monomers and TEGMA onTAB 2000®, is the highest, increasing until the 7th day anddecreasing afterwards until the 14th day.CONCLUSIONThe calibration of the HPLC method was achieved, with accurateretention times for each monomer. The studied molecules arereleased in toxic concentrations. TAB 2000® is made of MMA andTEGMA; Protemp 4TM is made of TEGMA and UDMA; and Structur3® is made of TEGMA, MMA and UDMA. TAB 2000® is the one withthe highest concentration of eluted monomers. The monomersrelease rate was modeled with two steps kinetic behavior, where theequilibrium is at the 7th day. In the first 24 hours the elution of themonomers is the highest.RP HPLC OF COMMON MONOMERS USED IN DENTAL RESINSREFERENCESBalkenhol, M., et al. (2007). Provisional crown and fixed partial denture materials: Mechanical properties and degree of conversion. Dental Materials, 23, 1574–1583.Chang, H. H., et al. (2014). Urethane dimethacrylate induces cytotoxicity and regulates cyclooxygenase-2, hemeoxygenase and carboxylesterase expression in human dental pulp cells. ActaBiomaterialia, 10, 722–731.Lee, D. H., et al. (2009). Effects of TEGDMA and HEMA on the expression of COX-2 and iNOS in cultured murine macrophage cells. Dental Materials, 25, 240–246.Polydorou, O., König, A., Hellwig, E., & Kümmerer, K. (2009). Long-term release of monomers from modern dental-composite materials. European Journal of Oral Sciences, 117, 68–75.Figure 1  - Optimization of the HPLC: analysis of five solutions at different concentrations of the monomers HEMA, TEGMA, MMA and UDMAy = 9,086175E+08x - 1,024175E+05R² = 9,918845E-010E+001E+062E+063E+064E+060E+00 1E-03 2E-03 3E-03 4E-03AUConcentration (M)y = 9,364644E+08x + 7,330625E+04R² = 9,989679E-010E+001E+062E+063E+064E+060E+00 1E-03 2E-03 3E-03 4E-03AUConcentration (M)y = 7,331143E+08x + 1,503625E+05R² = 9,913645E-010E+001E+062E+063E+064E+060E+00 1E-03 2E-03 3E-03 4E-03 5E-03AUConcentration(M)UDMAy = 5,269432E+06x - 2,367313E+04R² = 9,980729E-010E+005E+051E+062E+062E+060E+00 1E-01 2E-01 3E-01 4E-01AUConcentration (M)HEMATEGDMAMMACalibration curves obtained fromHPLC: analysis of the monomersHEMA, TEGMA, MMA and UDMA Figure 2 - HPLC chromatograms of released tosolution compounds of TAB2000®, Protemp4TM, Structur 3® resinsCHROMATOGRAMS OF RELEASED COMPOUNDSMONOMERS CALIBRATION CURVES

HPLC quantitative determination of acrylic monomers released from resins used in dental restoration

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HPLC quantitative determination of acrylic monomers released from resins used in dental restoration

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