ARVO Annual Meeting AbstractGlyn Chidlow, W. Chan, J. P. Wood, R. J. Casso

Casson, R.J.

Chan, W.

Chidlow, G.

Wood, J.P.

Investigative Ophthalmology & Visual Science

Wood, J.P.M.

Adelaide Research &amp; Scholarship

OPEN ACCESSARVO Annual Meeting Abstract  |   July 2019Glyn Chidlow; WengOnn Chan; John P M Wood; Robert James CassonAuthor Affiliations & NotesInvestigative Ophthalmology & Visual Science July 2019, Vol.60, 6603. doi:https://doi.org/Purpose : Rodents are increasingly used to study signaling pathways activated duringretinal detachment (RD), and, to test the effectiveness of putative neuroprotectivestrategies. Yet, relative to the extensive body of work conducted on cats, there existfewer data pertaining to the effects of RD on the different retinal cell types in rodents.Clinically, the fate of cones following RD is of particular importance. Data from catshave shown that RD induces differing effects on rods and cones. The goal of thecurrent study was to shed light on the responses of cone photoreceptors to RD in therat.Methods : RD was induced in the right eyes of Sprague-Dawley rats via subretinalinjection of sodium hyaluronate; left eyes served as untouched controls. Animals werehumanely killed between 1 and 28 days after surgery. Eyes were removed and eitherplaced in Davidson’s fixative and subsequently embedded in paraffin for transversesectioning of the retina, or, were fixed in neutral buffered formalin and dissected asretinal wholemounts. Tissue sections and retinal wholemounts were processed forsingle- or double-labeling immunohistochemistry using standard methodologies.Results : From wholemount quantifications, the number of S-cones during RD wasreduced to 12.9±4.2% (1 week), 7.3±2.6% (2 weeks) and 9.2±4.9% (4 weeks) of the totalrecorded in the intact retina. The number of M/L-cones during RD was 59.2±13.1% (1week), 24.4±8.4% (2 weeks) and 22.4±4.9.2% (4 weeks) of the intact retina. Fromanalysis of transverse sections, expression of peanut agglutinin (PNA), arrestin, andperoxiredoxin-1 in cones was largely unchanged at 1 day after RD. In contrast, mostS-opsin labelling was lost, M/L-opsin segments were typically short and distorted, whilstThe responses of cone photoreceptors to retinal detachment in the rat |... http://iovs.arvojournals.org/article.aspx?articleid=27455551 of 2 22/11/2019, 4:58 pmneuron-specific enolase in cones was reduced. Between 3 and 7 days after RD, cone“deconstruction” continued, with variably reduced expression of arrestin andperoxiredoxin-1, and disorganised and collapsed labelling of PNA. Cone markers werebroadly consistent between 1 and 4 weeks after RD. Of note, expression was oftenpatchy, likely being related to height of RD.Conclusions : Expression of S-opsin is lost earlier than M/L-opsin after RD in rats, whileother cone markers, notably PNA and arrestin, are more resilient. A significantproportion of cones survive for 4 weeks after RD. The present results have implicationsfor cone identification and quantification during experimental studies of RD.This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver,Canada, April 28 - May 2, 2019.This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives4.0 International License.The responses of cone photoreceptors to retinal detachment in the rat |... http://iovs.arvojournals.org/article.aspx?articleid=27455552 of 2 22/11/2019, 4:58 pm

The responses of cone photoreceptors to retinal detachment in the rat

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The responses of cone photoreceptors to retinal detachment in the rat

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The responses of cone photoreceptors to retinal detachment in the rat

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Adelaide Research & Scholarship