Abstract not availableThomas D. Michl, Carla Giles, Piotr Mocny, Kathryn Futrega, Michael R. Doran, Harm-Anton Klok, Hans J. Griesser, Bryan R. Coa

Coad, B.

Doran, M.

Futrega, K.

Giles, C.

Griesser, H.

Klok, H.

Michl, T.

Mocny, P.

Biointerphases

Adelaide Research &amp; Scholarship

Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans

Thomas D. Michl

Carla Giles

Piotr Mocny

Kathryn Futrega

Michael R. Doran

Harm-Anton Klok

Hans J. Griesser

Bryan R. Coad

Crossref

Caspofungin on ARGET-ATRP grafted PHEMA polymers: Enhancement and selectivity of prevention of attachment of<i>Candida albicans</i>

Free access via publisher's website. There is a need for coatings for biomedical devices and implants that can prevent the attachment of fungal pathogens while allowing human cells and tissue to appose without cytotoxicity. Here, the authors study whether a poly(2-hydroxyethylmethacrylate) (PHEMA) coating can suppress attachment and biofilm formation by Candida albicans and whether caspofungin terminally attached to surface-tethered polymeric linkers can provide additional benefits. The multistep coating scheme first involved the plasma polymerization of ethanol, followed by the attachment of α-bromoisobutyryl bromide (BiBB) onto surface hydroxyl groups of the plasma polymer layer. Polymer chains were grafted using surface initiated activators regenerated by electron transfer atom transfer radical polymerization with 2-hydroxyethylmethacrylate, yielding PHEMA layers with a dry thickness of up to 89 nm in 2 h. Hydroxyl groups of PHEMA were oxidized to aldehydes using the Albright–Goldman reaction, and caspofungin was covalently immobilized onto them using reductive amination. While the PHEMA layer by itself reduced the growth of C. albicans biofilms by log 1.4, the addition of caspofungin resulted in a marked further reduction by >4 log units to below the threshold of the test. The authors have confirmed that the predominant mechanism of action is caused by antifungal drug molecules that are covalently attached to the surface, rather than out-diffusing from the coating. The authors confirm the selectivity of surface-attached caspofungin in eliminating fungal, not mammalian cells by showing no measurable toxicity toward the myeloid leukaemia suspension cell line KG-1a

Michl, Thomas

Giles, Carla

Mocny, Piotr

Futrega, Katarzyna

Doran, Michael

Klok, Harm-Anton

Griesser, Hans

Coad, Bryan

Queensland University of Technology ePrints Archive

Caspofungin on ARGET-ATRP grafted PHEMA polymers: Enhancement and selectivity of prevention of attachment of Candida albicans

There is a need for coatings for biomedical devices and implants that can prevent the attachment of fungal pathogens while allowing human cells and tissue to appose without cytotoxicity. Here, the authors study whether a poly(2-hydroxyethylmethacrylate) (PHEMA) coating can suppress attachment and biofilm formation by Candida albicans and whether caspofungin terminally attached to surface-tethered polymeric linkers can provide additional benefits. The multistep coating scheme first involved the plasma polymerization of ethanol, followed by the attachment of a-bromoisobutyryl bromide (BiBB) onto surface hydroxyl groups of the plasma polymer layer. Polymer chains were grafted using surface initiated activators regenerated by electron transfer atom transfer radical polymerization with 2-hydroxyethylmethacrylate, yielding PHEMA layers with a dry thickness of up to 89 nm in 2 h. Hydroxyl groups of PHEMA were oxidized to aldehydes using the Albright-Goldman reaction, and caspofungin was covalently immobilized onto them using reductive amination. While the PHEMA layer by itself reduced the growth of C. albicans biofilms by log 1.4, the addition of caspofungin resulted in a marked further reduction by>4 log units to below the threshold of the test. The authors have confirmed that the predominant mechanism of action is caused by antifungal drug molecules that are covalently attached to the surface, rather than out-diffusing from the coating. The authors confirm the selectivity of surface-attached caspofungin in eliminating fungal, not mammalian cells by showing no measurable toxicity toward the myeloid leukaemia suspension cell line KG-1a. VC (C) 2017 American Vacuum Society

Michl, Thomas D.

Futrega, Kathryn

Doran, Michael R.

Griesser, Hans J.

Coad, Bryan R.

University of Queensland eSpace

Infoscience - École polytechnique fédérale de Lausanne

Free access via <a href="http://avs.scitation.org/doi/10.1116/1.4986054">publisher's website</a>.\ud
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There is a need for coatings for biomedical devices and implants that can prevent the attachment of fungal pathogens while allowing human cells and tissue to appose without cytotoxicity. Here, the authors study whether a poly(2-hydroxyethylmethacrylate) (PHEMA) coating can suppress attachment and biofilm formation by Candida albicans and whether caspofungin terminally attached to surface-tethered polymeric linkers can provide additional benefits. The multistep coating scheme first involved the plasma polymerization of ethanol, followed by the attachment of α-bromoisobutyryl bromide (BiBB) onto surface hydroxyl groups of the plasma polymer layer. Polymer chains were grafted using surface initiated activators regenerated by electron transfer atom transfer radical polymerization with 2-hydroxyethylmethacrylate, yielding PHEMA layers with a dry thickness of up to 89 nm in 2 h. Hydroxyl groups of PHEMA were oxidized to aldehydes using the Albright–Goldman reaction, and caspofungin was covalently immobilized onto them using reductive amination. While the PHEMA layer by itself reduced the growth of C. albicans biofilms by log 1.4, the addition of caspofungin resulted in a marked further reduction by >4 log units to below the threshold of the test. The authors have confirmed that the predominant mechanism of action is caused by antifungal drug molecules that are covalently attached to the surface, rather than out-diffusing from the coating. The authors confirm the selectivity of surface-attached caspofungin in eliminating fungal, not mammalian cells by showing no measurable toxicity toward the myeloid leukaemia suspension cell line KG-1a

Name not available

Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans

Abstract

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Full text

Available Versions

Adelaide Research & Scholarship

Crossref

Queensland University of Technology ePrints Archive

University of Queensland eSpace

Infoscience - École polytechnique fédérale de Lausanne

Name not available

Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans

Abstract

Similar works

Full text

Available Versions

Adelaide Research &amp; Scholarship

Crossref

Queensland University of Technology ePrints Archive

University of Queensland eSpace

Infoscience - École polytechnique fédérale de Lausanne

Name not available

Adelaide Research & Scholarship