II International Conference on Antimicrobial Research.
Abstract
Both Campylobacter and Salmonella are considered the most frequent bacterial causes of human enteritis in
industrialized countries. The consumption of raw or undercooked poultry and pork contaminated meat products
are the main sources of human infection. The prevalence of Campylobacter and Salmonella was determined in
the present work for extensive production Iberian pig sows, Sus mediterraneus. Samples were collected at the
maternity area of a creator from, water drinkers, feed and feed containers as well as from sows faecal matter. Of
42 samples, 31 and 23 carried Campylobacter spp. and Salmonella spp. respectively. Only Salmonella spp. was
found in all 3 tested water and feed containers. Of the 58 isolated Campylobacter strains only one was identified,
by multiplex-PCR, as Campylobacter jejuni, all other were C. coli. Antibiotic susceptibility was performed by
disc diffusion method with Nalidixic acid, Ciprofloxacin, Erythromycin, Tetracycline, Chloramphenicol and
Ampicilin. While 95% of the tested strains were susceptible to chloramphenicol, 66% and 53% were resistant to
the tested fluoroquinolones, Ciprofloxacin and Nalidixic acid respectively. Erythromycin resistance was fairly
low in comparison to previous publications with 14% of resistant strains. 38% were resistant to Tetracycline and
57% to Ampicilin. Seven of the 58 Campylobacter strains were entirely susceptible and none were resistant to all
the antimicrobials tested. Multiple drug resistance was found in 88% of strains. Cross contamination may occur
between sows inside maternity facilities and piglets may become infected in an early age by their mothers. New
and better control measures are therefore necessary to minimize transmission between animals reducing the
number of contaminated individuals and the potential transmission to human handlers and consumers
