During a collecting trip undertaken by J. Roháček and M. Vála in the São Miguel Island (Azores) in August and September 2006, mainly devoted to acalyptrate flies, three distinctive species of Diptera were found, two of which proved to be hitherto unrecorded from the Azorean archipelago. These additions to the regional fauna
are presented below with a discussion of their origin. The voucher specimens of the species recorded below are deposited in the following collections: JSO – collection of J. Starý, Olomouc, Czech Republic, SMOC – Silesian Museum, Opava, Czech Republic, ZMAN – Zoological Museum, Amsterdam, Netherlands