The use of stereolithography (SL) for fabricating complex three-dimensional (3D) tissue
engineered scaffolds of aqueous poly(ethylene glycol) (PEG) hydrogel solutions is described.
The primary polymer used in the study was PEG-dimethacrylate (PEG-dma) with an average
molecular weight (MW) of 1000 in distilled water with the photoinitiator Irgacure 2959 (I-2959).
Successful layered manufacturing (LM) with embedded channel architecture required
investigation of the photopolymerization characteristics of the PEG solution (measured as
hydrogel thickness or cure depth) as a function of photoinitiator concentration and laser energy
dosage for a specific photoinitiator type and polymer concentration in solution. Hydrogel
thickness was a strong function of PI concentration and energy dosage. Curves of hydrogel
thickness were utilized to successfully plan, perform, and demonstrate layered manufacturing of
highly complex hydrogel scaffold structures, including structures with internal channels of
various orientations. Successful fabrication of 3D, multi-layered bioactive PEG scaffolds
containing cells was accomplished using a slightly modified commercial SL system (with 325
nm wavelength laser) and procedure. Human dermal fibroblast (HDF) cells were encapsulated in
PEG hydrogels using small concentrations (~ 5 mg/ml) of acryloyl-PEG-RGDS (MW 3400)
added to the photopolymerizable PEG solution to promote cell attachment. HDF cells were
combined with the PEG solution, photocrosslinked using SL, and successfully shown to survive
the fabrication process. The combined use of SL and photocrosslinkable biomaterials such as
PEG makes it possible to fabricate complex 3D scaffolds that provide site-specific and tailored
mechanical properties (i.e., multiple polymer materials) with a polymer matrix that allows
transport of nutrients and waste at the macroscale and facilitates cellular processes at the
microscale through precisely placed bioactive agents.Mechanical Engineerin