2001-2002 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Chau, FT

Gong, F

Leung, AKM

Liang, YZ

Peng, YG

Song, YQ

PolyU Institutional Repository

??????????? (Ⅱ )—— ?????? GC /MS??龚　范1　梁逸曾1　宋又群2　彭源贵3　 Leung Alexander Kai-man4　 Chau Foo-tim4( 1. ?????????? , ?????????? , ?? 410083; 2. ?????????? ,??????????????? , ?? 410082; 3. ??????????????? ; ?? 410013;4. ???????????????? , ?? , ?? )??　?????? ( GC /M S)???????????????????????????? , ????????????? ( HELP)????????? /???????? , ??????????????? ,???????????????????????????????? , ?????????????? . ????? 98??? , ????? 44??? , ????????? 58. 74% .???　 GC /MS; ???????????? ; ??????????　 O657　　　　?????　 A　　　　????　 0251-0790( 2001) 09-1481-05????: 2000-07-20.????: ???????? (???: 29875007)?? .?????: ??? ( 1950??? ) ,? , ?? , ?? , ????? ,????????? .?? (Cortex Magnoliae Of f icinalis )???????????????? , ??????????????????、 ????? , ?????????????? , ???????????? [1 ] ,???????????????? , ??????????????? , ????????????????????????? [2 ] . ?? , ???????????????????? , ???????????????????????? . ?? [3, 4 ]?? GC /M S???? HELP?????????? /???? , ????????????????????????????????? ,??????????????? , ??????????????????? , ??????????? . ???? HELP??????????????????? , ????????? .1　????1. 1　?????GC-17A Shimadzu????? , Qp-5000 Shimadzu??? . ?????????????? , ????????????????? .1. 2　????????? 200 g????? , ??????????? ( 1995?? )??????????? .1. 3　????????????: OV-17?????? 30 m× 0. 25 mm. ????: ( 1)???? 60℃ , ?? 2 min, ?20℃ /min?? 270℃ , ?? 20 min; ( 2)???? 60℃ , ? 10℃ /min?? 150℃ , ?? 3℃ /min?? 200℃ , ??? 10℃ /min?? 230℃ ,?? 5 min; ( 3) ???? 60℃ , ? 5℃ /min?? 230℃ , ?? 5 min; ??: He; ??: 1? 0. 5 mL /min; ????: 250℃ .????: EI????? 70 eV; ?????: 230℃ ; ????: 1. 10 kV; ????: 20～350 amu; ????: 0. 2 s /? .Vol. 22 高 等 学 校 化 学 学 报 　 No. 9　2 0 0 1? 9? 　 　　　　　　 CHEM ICAL JOURN AL O F CHINE SE UN IVERSITIES　　 　　　　　　　 1481～ 1485　2　?????2. 1　????????????Fig. 1　 Total ionic chromatography of chemical compo-nents in the volatile oil f rom Cortex MagnoliaeOf f icinalis under condition ( 1) for programedtemperature? 1???????? ( 1)、?? 1 mL /min?????????? GC /M S??????? , ?? 50???? , ?????????? , ????9. 96～ 13. 67 min??? (? 3 001～ 4 100????? )???????? . ?? , ? HELP???????????????? , ??????????????? , ????????????????????? , ????????????????? . ? 10. 20～ 10. 40 min? ???? (?3 061～ 3 110????? )????? A??? .? 2??? A?????? ,???????????? , ????????? 2-pentadecyn-1-ol?D-nerolidol. ?? , ??? HELP????????? /???? , ???????????? 5????? (????? 1— 5) . ??????? HELP??????????????? /???????? .?? 1?? , ????????? , ?????? , ??????????????? , ??????????? . ?? , ????????????????? , ?? [5～ 9 ]?????? . ???????????????? .Fig. 2　 Total ionic chromatography within 10. 20— 10. 40min( 3 061— 3 110 scan points)Fig. 3　 Evolving latent projection graph of chemicalcomponents in the peak cluster A? 3??????????? ( Evolving latent projection g rapth, ELPG) . ??????? , ?????? , ?????? 5??? , ?? 3????????? 1— 5????? . ?????????? ( Eigenst ructure t racking analysis, ET A)???? ( Rankmap) [5～ 9 ]????????????????? , ?? 3???????????????? . ???? , ???????????????1, ?????????? , ?? 2, ???????? , ???? . ? 4?????? , ???? , ?3?????????????? , ??? 1, ??????? 1, ???? 1????? ; ???1+ 2, ??????? 2, ??????? 1????????????? 2????? , ??? 1+2??????? ; ??? 1+ 2+ 3, ??????? 3, ????? 1? 2?????????? 3????? , ??? 1+ 2+ 3??????? ; ??? 2+ 3, ??????? 2, ????? 2+ 3??????? ; ??? 2+ 3+ 4, ??????? 3, ????? 2+ 3+ 4??????? ; ???3+ 4, ??????? 2, ??? 3+ 4??????? ; ??? 4, ??????? 1, ??? 4????? ; ??? 4+ 5, ??????? 2, ??? 4+ 5??????? ; ??? 5, ???????1, ???? 5, ???? 5????? .???? , ? 10. 20～ 10. 40 min????????????? , ??????????????1482　　　 高 等 学 校 化 学 学 报 Vol. 22???????????、 ????????、 ???????? (???? )?????? (??????????? )???????? . ?????????、 ??????????????? , ??????????????????????????? .Fig. 4　 Rankmap of chemical componentsin peak cluster AFig. 5　Resol ved chromatograms of chemicalcomponents in peak cl uster A??????????????? , ????????????????? , ??????????????????? , ?????? 1, 2, 4? 5??? Ledane, Palustrol, 2-Pentadecyn-1-ol?D-nerolidol, ????????????? 5, ???? 6—? 9, ?? 3????????????? . ?? 5—? 9?? , 5?????????????????? , ??????????? , ?? ,?????????????????? , ????????????????????? .Fig. 6　 Resolved mass spectrum( A) of component 1 andthe standard mass(B) of ledane(C15H26 )Fig. 7　 Resolved mass spectrum (A) of component 2 andthe standard mass(B) of pal ustrol(C15H26O)　 Fig. 8　 Resolved mass spectrum (A) of component 4 andthe standard mass(B) of 2-pentadecyn-1-ol( C15H28O)　 Fig. 9　Resol ved mass spectrum (A) of component 5and the standard mass(B) of D-nerolidol(C15H26O)??????? , ??????????????? , ??????????? , ????????????????????? , ?????????? 1. ?????? , ????????????? , ???????? (? 62 000????? )????????? , ??????????? , ???????? , ????????????????? . ? HELP?????????????????????????? , ???????????????? . ????????????? , ??????????????????????????????????? . ?? , ??1483No. 9 龚　范等: 平胃散挥发性成分的研究 (Ⅱ ) 　　　??????????????????????????????? .Table 1　 The qual itat ive and quantitative analyses results of some chemical components of the vol atil e oil5 tR / s Name of componen t　　　　　　　　　　 Molecular formula Rel. Con. (% )958 n-Hex anal C6H12O 0. 031 196 cis-Ocimene C10H16 0. 571 295 Camphene C10H16 0. 171 408 U-Pinene C10H16 0. 191 496 2-Pentylfuran C10H16 0. 091 567 D-Limon ene C10H16 0. 951 658 m-Cymene C10H14 1. 081 666 1, 8-Cineole C10H18O 0. 411 798 trans-Linaloloxide C10H18O2 0. 191 846 3, 7-Dimethyl-1, 6-octadien-3-ol C10H18O 0. 322 014 2-Ph enylethanal C8H8O 0. 042 051 n-Octoic acid C8H16O2 0. 602 156 L -Borneol C10H18O 0. 252 180 Myrcenol C10H18O 0. 102 199 T-Terpineol C10H18O 0. 502 225 [R ]-[+ ]-Cit ronellal C10H18O 0. 052 264 U-Cit ronellal C10H18O 0. 052 311 [+ ]-V erbenol C10H16O 0. 112 336 cis-Geraniol C10H18O 0. 162 420 Limonene monooxide C10H16O 0. 052 505 T-Phen ylallyl alcohol C9H10O 0. 662 645 Isocaryophyllen e C15H24 0. 082 743 T-Humulen e C15H24 0. 052 821 U-Elemene C15H24 0. 692 868 cis-U-Farnesen e C15H24 0. 042 902 3, 3, 7, 7-Tertrameth yl-5-[2-methyl-1-propenyl ]-tricyclo [4. 1. 0. 0]-2, 4-h eptane C15H24 0. 142 949 4, 11, 11-Trimethyl-8-meth ylene-bicyclo [ 7. 2. 0 ] undec-4-ene C15H24 0. 062 977 Dodecanoic acid C12H24O2 0. 252 999 Elemol C15H24 0. 393 021 Patchulane C15H24 0. 183 072 Ledan e C15H26 0. 253 078 Palust rol C15H26O 0. 723 094 2-Pentadecyn-1-ol C15H28O 0. 843 098 D-Nerolidol C15H26O 0. 283 146 Dihydrocarveol acetate C12H20O2 5. 453 168 T-Elemene C15H24 0. 723 184 T-Fran esene C15H24 0. 763 210 1, 5, 5, 8-Tetrameth yl-12-oxabicyclo [9. 1. 0 ] dodeca-3, 7-dien e C15H24O 3. 383 289 U-Eudesmol C15H26O 15. 563 400 Cadalene C15H18 0. 693 454 n-Pentadecanoic acid C15H30O2 1. 103 637 n-Hex adecanoic acid C16H32O2 12. 193 950 n-Nonad ecanoic acid C19H38O2 1. 214 004 Methyl linolelaidate C19H34O2 7. 702. 2　????????????????? [9 ]?????? GC /M S??????? , ??????????????? , ?????????????????? , ?????? GC /M S??????????????????? , ??????????? . ???????? 1? .?????????????? HELP???????????? , ??????????、 ???????????????????????????????? (??????? 2??? ,??? 10???????? 2?????????? , A??? 1????? A?? ) . ???? ,?????? , ????????????? . ???? HELP???? 10?? A?? , ????????????? , ?? 1????? A?????? . ????????????? , ????1484　　　 高 等 学 校 化 学 学 报 Vol. 22Fig. 10　 Total ionic chromatography of chemical compo-nents in the vol atil e oil f romCortex MagnoliaeOf f icinalis under condit ion ( 2) f or programedtemperature??????? . ?? , ?? HELP?????????????????????????? , ???????????????????? .???????????????? , ?HELP??????? /???? , ?????????? , ??????????????? , ?????? GC /M S???????????????????????????????????? , ????????? . ?? , ??????????????????????????? .?　?　?　?　 1　 LI Jia-Shi(??? ) . Iden tifi cation Science of Tradi tional Chines e M edicine(????? ) [M ] , Shanghai: Shanghai Publishing Houseof Science and Tech nology, 1996: 409— 413　 2　 ZEN G Quan(?　? ) , ZHOU Gan-Nan (??? ) , TU Wan-Qian(??? ) et al . . Chinese Tradi tional and Herbal Drugs (??? )[ J] , 1997, 28: 81— 83　 3　 GONG Fan (?　? ) , SON G You-Qun (??? ) , PENG Yuan-Gui (??? ) et al . . Acta Ph armaceutica Sinica(???? ) [ J ] ,2000, 35: 394— 396　 4　 GONG Fan(?　? ) , LIANG Yi-Zeng(??? ) , SON G You-Qun (??? ) et al . . Chinese Journal of Analytical Chemis try(???? ) [ J] , 2000, 28: 860— 864　 5　 LIAN G Yi-Zeng(??? ) . White, Grey and Black M ulticomponent Systems and Th eir Chemomet rics Algori thms(????????????????????? ) [M ] , Changsha: Hunan Publishing House of Science and Tech nology, 1996: 177— 206　 6　 Kvalh eim O. M . , LIAN G Y. Z. . Anal. Chem. [ J ] , 1992, 64: 936— 945　 7　 LIAN G Y. Z. , Kvalheim O. M . , Keller H. R. et al . . Anal. Chem. [ J] , 1992, 64: 946— 953　 8　 GONG Fan(?　? ) , PENG Yuan-Gui (??? ) , CU I Hui (?　? ) et al . . Acta Ph armaceutica Sinica(???? ) [ J ] , 1999, 34:214— 217　 9　 GONG Fan(?　? ) , PENG Yuan-Gui (??? ) , C UI Hui (?　? ) et al . . Chem. J. Chinese Universi ties(???????? )[ J] , 1999, 20( 2): 199— 203Determination of Volatile Components of Ping-wei Powder(Ⅱ )—— Analysis of the Volatile Oil inCortex Magnol iae Of ficinalis with GC /MSGONG Fan1 , L IANG Yi-Zeng1* , SON G You-Qun2 , PEN G Yuan-Gui3Leung Alexander Kai-man4 , Chau Foo-tim4( 1. College of Chemistry and Chemical Engineering , Research Centre of Chinese Traditional and Herbal Drug Moderniza-tion, Central South University , Changsha 410083, China; 2. College of Chemistry and Chemical Engineering , Institute ofChemometrics and Chemical Sensing Technology , Hunan University , Changsha 410082, China; 3. Institute of MateriaMed ica, Hunan Academy of Traditional Chinese Medicine & Materia Medicai , Changsha 410013, China; 4. Departmemtof Applied Biolog y and Chemical Technolog y , The Hongkong Poly technic Universit y , Kowloon, Hongkong , China)Abstract　 This paper aims at detecting chemical components of the volatile oil inCortex Magnoliae O f f ici-nalis with GC /M S. With the help of heuristic ev olving latent projections( HELP) resolution and overallvolume integ ration method upon two-w ay data, 98 components are separated and 44 of them qualita tivelyand quantitatively analy zed representing about 58. 74% of the total content.Keywords　 GC /M S; Help of heuristic ev olving latent projections; Volatile oil inCortex Magnoliae O f f ici-nalis ( Ed.: K, G)1485No. 9 龚　范等: 平胃散挥发性成分的研究 (Ⅱ ) 　　　

Determination of Volatile Components of Ping-wei Powder(II) - Analysis of the Volatile Oil in Cortex Magnoliae Officinalis with GC/MS

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