Alonso-Orgaz, Sergio

Barderas, María G.

Gil-Dones, Félix

Laborde, Carlos M.

Moreu, José

Mouriño-Álvarez, Laura

Rodríguez-Padial, Luis

Vivanco, Fernando

Repositorio Institucional de la Universidad de Córdoba

S6. Biomarker Discovery and Validation Poster P65 176  CHARACTERIZATION OF INDIVIDUALIZED PROTEOMIC PROFILES IN ST-SEGMENT ELEVATION AND NON ST-SEGMENT ELEVATION ACUTE CORONARY SYNDROME C. M. Laborde (1), L. Mouriño (1), S. Alonso-Orgaz (1), J. Moreu (2), L. R. Padial (2), F. Vivanco (3), F. Gil-Dones (1), M. G. Barderas (1). (1) Hospital Nacional de Parapléjicos, (2) Hospital Virgen de la Salud, (3) IIS-Fundación Jiménez Díaz. INTRODUCTION: Acute Coronary Syndrome (ACS) is one of the main causes of morbidity and mortality in developed countries. Despite the research advances in recent years, ACS prevention and treatment strategies still suffer from significant limitations therefore new theoretical and technical approaches are required. The application of proteomics to ACS research constitutes an invaluable tool for the searching of new disease mechanisms, novel biomarkers and therapeutic targets, as well as to increase our understanding of the origin and development of this syndrome. In this work, we focused in the establishment of an individualized proteomic profile in non ST-segment elevation ACS (NSTE-ACS) and ST-segment elevation ACS (STE-ACS) using two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry (MALDI-TOF/TOF). MATERIAL AND METHODS: EDTA plasma samples of 20 patients with NSTE-ACS, 20 patients with STE-ACS and 20 healthy controls recruited from the cardiology service of Hospital Virgen de la Salud were collected for this study. 2D-DIGE experiments were carefully designed for STE-ACS vs healthy controls (n=6) and for NSTE-ACS vs healthy controls (n=5). Previously, all plasma samples were depleted using a Multi Affinity Removal column (MARS Hu-14, Agilent Technologies). Selected reaction monitoring (SRM) and immunoblotting were used for the validation process. RESULTS: 2-D Differential analysisrevealed 24 differentially expressed spots in plasma of NSTE-ACS patients (12 upregulated, 12 downregulated) and 47 in STE-ACS (13 upregulated, 34 downregulated). The identification step is currently being completed and several of the identified proteins have already been validated. CONCLUSIONS: Our proteomic 2D-DIGE experiments demonstrate that STE-ACS and NSTE-ACS can be defined by different and individualized proteomic profiles. These results could illuminate the understanding of changes implicated in the atherosclerotic process in both cases. We also expect to identify interesting proteins, which could be used as novel potential biomarkers for the prognosis and/or treatment of ACS in clinical practice. 

Characterization of individualized proteomic profiles in ST-segment elevation and no ST-segment elevation acute coronary syndrome

Characterization of individualized proteomic profiles in ST-segment elevation and no ST-segment elevation acute coronary syndrome

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Repositorio Institucional de la Universidad de Córdoba