Albar-Ramírez, Juan Pablo

Albizua, E.

Ayala, R.

Barrio, S.

Candelas, M.

Fernández, M.

Gallardo, M.

García, V.

Jiménez, A.

Martínez-López, J.

Paradela, Alberto

Puigdecanet, E.

Redondo, S.

Repositorio Institucional de la Universidad de Córdoba

S6. Biomarker Discovery  Poster P96 210  PROTEIN PROFILE IN CHRONIC MYELOPROLIFERATIVE NEOPLASMS (MPN). M. Gallardo(1), S. Barrio(1), E. Albizua(1), R. Ayala(1), A. Jiménez(1), M. Candelas(1), M. Fernández(1), A. Paradela(2), J.P. Albar(2), E. Puigdecanet(3), S. Redondo(4), V. García(5) y J. Martínez-Lopez(1). (1)Servicio de Hematología, Hospital 12 de Octubre, Madrid. (2)Servicio de proteómica, CNB, Madrid. (3)Laboratori de Citogenètica Molecular, Hospital del Mar, Barcelona, (4)Servicio de Hematologia, Hospital Clínico, Madrid, (5)Servicio de Hematologia, Hospital Ramon y Cajal, Madrid AIMS: Perform a genomic and proteomic analysis in myeloproliferative neoplasms (MPN), essential thrombocythemia (ET) and Polycythemia Vera (PV) that can determine the origin and divergence physiopathogenic of both diseases. MATERIALS AND METHODS: Studies consisted of 68 samples in total, including 52 patients, 26 ET JAK2V617F positive and 26 PV and the remaining 16, healthy donors. Peripheral blood neutrophils (SP) were used in all experiments. cDNA was used for quantitative PCR of the gene MMP14. PCR results were validated by flow cytometry using anti-MMP14 and anti-CD45 antibodies. We used the cytosolic protein fractions of these cells for a screening by 2D-DIGE gels and subsequent mass spectrometry. Statistical analysis of the results of quantitative PCR was performed using the Wilcoxon test (p-value <0.05). DeCyder v 7.0 software was used (GE) for the analysis of 2D-DIGE gels and Mascot for MS. RESULTS: MMP14 showed a differential expression between PV and ET populations (over-expressed in PV) regardless of treatment (p-value: 0.001). Preliminary analysis by flow cytometry suggest aberrant expression of MMP14 in PV and ET neutrophils compared with healthy donors. 2D-DIGE gels and mass spectrometry revealed the MMP9 protein overexpressed in ET compared to the PV positive. Other differentially expressed proteins were found in both populations, like HSPA8  and HSPA1A chaperones. CONCLUSIONS: Along with other metalloproteases, MMP14 and MMP9 may be involved in phenotypic divergence of PV and ET as well as in the pathogenesis of NMP-philadelphia negative.

Protein profile in chronic myeloproliferative neoplasms (MPN)

Protein profile in chronic myeloproliferative neoplasms (MPN)

Abstract

Similar works

Full text

Available Versions

Repositorio Institucional de la Universidad de Córdoba