García-Barrera, Tamara

González-Fernández, M.

Gómez-Ariza, José Luis

Jurado, Juan

López-Barea, Juan

Pueyo, Carmen

Repositorio Institucional de la Universidad de Córdoba

S6. Animal, Plant and Microbial Proteomics Posters166P. 94INTEGRATION OF PROTEOMICS WITH TRANSCRIPTOMICS AND METALLOMICS FOR THE STUDY OF ENVIRONMENTAL ISSUESJ.L. Gómez-Ariza1, M. González-Fernández1, T. García-Barrera1, J. Jurado2, J.López-Barea2, C. Pueyo21Dpto. de Química y CC.MM. Facultad de Ciencias Experimentales. Universidad de Huelva. Campus de El Carmen.21007 Huelva (Spain); 2Dpto. de Bioquímica y Biología Molecular. Campus Universitario de Rabanales. Edificio Severo Ochoa (C-6), 2ª planta. Ctra Madrid-Cádiz Km 396a, 14071 Córdoba (Spain)Metal bound to biomolecules play important roles in living organisms. The identification of these metal-biomolecules in biological tissues can be performed by multidimensional methods combining orthogonal chromatographic with mass spectrometry and inductively coupled plasma mass spectrometry (ICP-MS): METALLOMICS. The integration of these analytical methods with the proteomic approach provides a powerful tool in deciphering environmental issues related to metal action. This integrated approach has an special interest when the genetic homology between model organisms and test or bioindicator organisms is demonstrated, because important conclussions can be drawn using model organisms as pattern in environmental and health studies based on non-model (non-sequenced) ones (1).Present study is based on the use of M. musculus the best-known vertebrate model organism, and M. spretus the best characterized aboriginal species. Recently, M. spretus is being used as bioindicator in environmental pollution studies, through the use of different cytogenetic and biochemical biomarkers. Sequences of Cyp and Gst genes from Mus musculus were obtained from GenBankTM. Designed primer pairs based on M. musculus amplified in M. spretus induce products exhibiting in most cases 100% nucleotide sequence identity with M. musculus. The application of 2-DE protein separation and high throughput MALDI-TOF-PMF analysis assess the biological effect of polluted terrestrial ecosystems on inhabitant M. spretus mice. Spot identification relied on peptide matching with available sequence databases from M. musculus. Remarked differences are tested in the proteome of animals dwelling at different polluted environments, with specific increases and decreases in selected groups of proteins and changes that seem to be co-ordinately regulated.For the first time the molecular size distribution patterns of elements in Mus musculus mice has been obtained using the SEC-UV-ICP-MS approach. The profiles of the elements obtained make possible deep insight into the interactions of elements with the different organs of Mus musculus as well as their possible connection with the metabolic pathways in this animal. ICP-MS allows multielement profiling in samples to be obtained in only one chromatographic run, which constitutes a reliable technique Posters S6. Animal, Plant and Microbial Proteomics167P. 94with high throughput. A second chromatographic dimension on SEC-ICP-MS extracts usinf RP-HPLC-ICP-MS allows metallo-biomolecules purification and metal-species isolation for further identification by mass spectrometry techniques.Therefore, the following conclusions can be drawn:•  A genetic homology between Mus musculus and Mus spretus has been established•  Modification of proteins expression in Mus spretus was defined in connection with differentially polluted ecosystems•  Metallomics allows high throughput metal-bound to proteins tracing in different tissues from Mus musculus and integration with proteomics and transcriptomics results can be drawn.References: Gómez-Ariza, J.L., et al, Anal. Chim. Acta (2004) 524:15-22.Acknowledgement: Ministerio de Educación y Ciencia. Grant: CTM2006-08960-C02-01.

Integration of proteomics with transcriptomics and metallomics for the study of environmental issues

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